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Assay-kits

SensoLyte® 520 MMP-12 Assay Kit Fluorimetric - 1 kit

$554.00
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  • Cat.Number : AS-71157
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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. MMP-12 (macrophage elastase) is expressed in activated macrophages and may play a role in smoke-induced emphysema.
SensoLyte® 520 MMP-12 Assay Kit uses a 5-FAM (fluorophore) and QXL520™ (quencher) labeled FRET peptide substrates for continuous measurement of the enzyme activities. This substrate showed excellent specificity to MMP-12 with minimal cross-reaction with MMP-1, 2, 7, 8, 9, 9, 13 and 14. In an intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520™. Upon the cleavage of the FRET peptide by MMP-12, the fluorescence of 5-FAM is recovered, and can be continuously monitored at excitation/emission = 490 nm/520 nm. With superior fluorescence quantum yield and longer emission wavelength, 5-FAM/QXL520™ based FRET peptide is less interfered by the autofluorescence of test compounds and cellular components and provides better assay sensitivity.

Specifications

Packaging
Kits components
  • Component A: MMP-12 substrate Ex/Em=490 nm/520 nm upon cleavage: 60 µL Component B: 5-FAM-Pro-Leu-OH, Fluorescence reference standard Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Organic mercury. Handle with care! Do not dispose into sink!): 1 M, 20 µL Component D: Assay buffer: 20 mL Component E: Stop solution: 10 mL
Chemistry
UniProt number
  • P39900
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 10 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

The endogenous protease inhibitor TIMP-1 mediates protection and recovery from cutaneous photodamage

J Invest Dermatol. . 2012 Jun 21 ; 132(12) 2800 | DOI : 10.1038/jid.2012.204

  • U. Yokose

Matrix metalloproteinase-12 (MMP-12) SNP affects MMP activity, lung macrophage infiltration and protects against emphysema in COPD

Thorax . 2011 Jul 05 ; 66(11) 970 | DOI : 10.1136/thx.2011.159087

  • I. Haq

Modulation of the angiogenic phenotype of normal and systemic sclerosis endothelial cells by gain-loss of function of pentraxin 3 and matrix metalloproteinase 12

Arthritis Rheumatol . 2010 Aug 01 ; 62(8) 2488 | DOI : 10.1002/art.27522

  • F. Margheri

Avenanthramide C as a novel candidate to alleviate osteoarthritic pathogenesis.

BMB Rep. . 2021 Oct 03 ; 54(10) 528 | DOI : 10.5483/BMBRep.2021.54.10.108

  • TT. Tran
  • et al