IMG-EN-AS-71129-01.jpg
Assay-kits

SensoLyte® 490 MMP-2 Assay Kit Fluorimetric - 1 kit

$804.00
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  • Cat.Number : AS-71129
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated proteins capable of digesting extracellular matrix components. The importance of MMPs in tumor development and invasion is well known. MMP-2 (gelatinase A) is proposed as a potential therapeutic target.
The SensoLyte® 490 MMP-2 Assay Kit is a complete assay system designed to continuously analyze MMP activities or to screen MMP-2 inhibitors by using a fluorogenic MMP-2 substrate.
Members of the MMP family have poor substrate sequence specificity, making it difficult to use a peptide substrate alone to differentiate the activity of a particular MMP from other MMPs. If several MMPs are coexisting in your samples and you would like to specifically measure MMP-2 activity, please choose the SensoLyte® Plus MMP-2 Assay Kit, Cat# AS-72224.

Specifications

Packaging
Kits components
  • Component A: MMP-2 substrate EDANS/DABCYL FRET peptide Ex/Em=340 nm/490 nm upon cleavage: 270 µL Component B: EDANS, fluorescence reference standard Ex/Em=340 nm/490 nm: 1 mM DMSO solution, 10 µL Component C: APMA, 4-aminophenylmercuric acetate (Caution: Contains organic mercury and must be handled with care. Dispose according to local regulations): 1 M, 100 µL Component D: Assay buffer: 60 mL Component E: Stop solution: 30 mL
Chemistry
UniProt number
  • P08253
Properties
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Storage & stability
Storage Conditions
  • Store all components at -20°C. Protect components A and B from light. Components D and E can be stored at 4 °C for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Structure-function studies on inhibitory activity of Bungarus multicinctus protease inhibitor-like protein on matrix metalloprotease-2, and invasion and migration of human neuroblastoma SK-N-SH cells.

Toxicon. . 2009 Aug 23 ; 55(2-3) 353 | DOI : 10.1016/j.toxicon.2009.08.012

  • W. Chou

ERK1/2 activation induced by inflammatory cytokines compromises effective host tissue integration of engineered cartilage

Tissue Eng Part A . 2008 Jan 01 ; 15(10) 2825 | DOI : 10.1089/ten.TEA.2008.0663

  • F. Djouad

Chlorotyrosine promotes human aortic smooth muscle cell migration through increasing superoxide anion production and ERK1/2 activation

Atherosclerosis. . 2008 Feb 20 ; 201(1) 37 | DOI : 10.1016/j.atherosclerosis.2007.12.049

  • H. Mu

The inhibitory effects of silver diamine fluoride at different concentrations on matrix metalloproteinases

Dent Mater. . 2012 May 12 ; 28(8) 903 | DOI : 10.1016/j.dental.2012.04.011

  • M.L. Mei

Homocysteine-Enhanced Proteolytic and Fibrinolytic Processes in Thin Intraluminal Thrombus and Adjacent Wall of Abdominal Aortic Aneurysm: Study In Vitro.

Biomed Res Int . 2018 Dec 12 ; 2018 3205324 | DOI : https://doi.org/10.1155/2018/3205324

  • A. Siennicka
  • et al

4F2hc-silencing impairs tumorigenicity of HeLa cells via modulation of galectin-3 and β-catenin signaling, and MMP-2 expression.

Biochim Biophys Acta . 2013 Sep 01 ; 1833(9) 2045 | DOI : https://doi.org/10.1016/j.bbamcr.2013.04.017

  • A. Santiago-Gómez
  • et al