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Assay-kits

SensoLyte® Cell Viability and Proliferation Assay Kit Fluorimetric, Large size - 1 kit

$765.00
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  • Cat.Number : AS-71301
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Lactate dehydrogenase (LDH) exists ubiquitously in the cytoplasm of living mammalian cells and the measurement of cytoplasmic LDH activity is a well-accepted assay to quantify living cell numbers and indicate cell viablity. The DHL™ Cell Viability and Proliferation Assay Kit provides researchers with a convenient one-solution and one-step assay to count living cells in a culture and continuously monitor cell proliferation over time by measuring cytoplasmic LDH activity. In this kit, resazurin is used as a sensitive fluorogenic indicator. Resazurin is be converted to the strongly fluorescent resorufin (Ex/Em=560nm/590 nm) by cytoplasmic LDH. The kit can detect as few as 48 living cells with a linear range up to 5X104 cells (r2=0.99). This kit can also be used for high throughput screening of cell proliferation or cytotoxicity effect of a variety of compounds. 384-well or 1536-well format can be used with minor modifications. Kit Size: 10,000 assays

Specifications

Packaging
Kits components
  • Component A: Assay solution: 20 mL X 10 bottles
Properties
Absorbance (nm)
  • 560
Emission (nm)
  • 590
Storage & stability
Storage Conditions
  • Store all components at -20°C and protect from light.
Activity
Application
Detection Method
Detection Limit
  • 48-50000 cells
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Targeting neuropilin-1 in human leukemia and lymphoma

Blood . 2010 Nov 09 ; 117(3) 920 | DOI : 10.1182/blood-2010-05-282921

  • K. Karjalainen

IGF-1 increases invasive potential of MCF 7 breast cancer cells and induces activation of latent TGF-β1 resulting in epithelial to mesenchymal transition

Cell Commun Signal . 2011 May 02 ; 9 10 | DOI : 10.1186/1478-811X-9-10

  • L.A. Walsh

Comparison between adenoviral and retroviral vectors for the transduction of the thymidine kinase PET reporter gene in rat mesenchymal stem cells

J Nucl Med . 2008 Nov 05 ; 49(11) 1836 | DOI : 10.2967/jnumed.108.052175

  • V. Roelants

Signaling pathways involved in cyclooxygenase-2 induction by hepatocyte growth factor in non-small cell lung cancer

Mol Pharmacol. . 2020 Mar 19 ; 72(3) 769 | DOI : 10.1124/mol.107.034215

  • J.M. Siegfried