IMG-EN-AS-71127-01.jpg
Assay-kits

SensoLyte® 490 HIV Protease Assay Kit Fluorimetric - 1 kit

$804.00
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  • Cat.Number : AS-71127
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HIV protease (PR) is identified as an important drug-screening target for the design of selective acquired immunodeficiency syndrome (AIDS) therapeutics. The SensoLyte® 490 HIV Protease Assay Kit uses an optimized FRET peptide substrate for a continuous measurement of HIV protease activities. This FRET-based fluorogenic substrate is derived from a natural processing site for HIV-1 PR. Incubation of the recombinant HIV-1 PR with this substrate results in specific cleavage and a time-dependent increase in fluorescence intensity that is linearly related to the extent of substrate hydrolysis. Because of its simplicity and precision in the determination of reaction rates required for kinetic analysis, this kit offers many advantages over the commonly used HPLC or electrophoresis-based assays for peptide substrate hydrolysis by retroviral PRs.

Specifications

Packaging
Kits components
  • Component A: HIV-1 protease substrate EDANS/DABCYL FRET peptide Ex/Em=340nm/490nm upon cleavage: 600 µL Component B: EDANS, fluorescence reference standard: 100 µM DMSO solution, 20 µL Component C: Pepstatin A: 27.4 µg powder Component D: 2X Assay buffer: 50 mL Component E: Stop solution: 30mL Component F: DMSO: 100 µL Component G: DTT: 1 M, 200 µL
Properties
Absorbance (nm)
  • 340
Emission (nm)
  • 490
Storage & stability
Storage Conditions
  • Store all components at -20°C. Components E and F can be stored at room temperature for convenience. Protect Components A and B from light.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

HIV protease: Multiple fold inhibition by silver nanoparticles—Spectrofluorimetric, thermodynamic and kinetic analysis

J Taiwan Inst Chem Engin . 2014 Jul 01 ; 45(4) 1140 | DOI : https://doi.org/10.1016/j.jtice.2014.01.008

  • C.Y. Shing

Selective and facile assay of human immunodeficiency virus protease activity by a novel fluorogenic reaction

Anal Biochem . 2010 Feb 15 ; 397(2) 197 | DOI : 10.1016/j.ab.2009.10.030

  • Z. Yu

Increased expression and immunogenicity of HIV-1 protease following inactivation of the enzymatic activity

Vaccine . 2010 Nov 23 ; 29(4) 839 | DOI : 10.1016/j.vaccine.2010.10.083

  • D. Hallengärd

A method to simultaneously monitor hepatitis C virus NS3 helicase and protease activities.

Methods Mol Biol . 2010 Jan 01 ; 587 223 | DOI : 10.1007/978-1-60327-355-8_16

  • D.N. Frick

HIV-1 Protease as DNA Immunogen against Drug Resistance in HIV-1 Infection: DNA Immunization with Drug Resistant HIV-1 Protease Protects Mice from Challenge with Protease-Expressing Cells

Cancers . 2023 Jan 01 ; 15(1) 238 | DOI : https://doi.org/10.3390/cancers15010238

  • S. Petkov
  • et al