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Peptides  >  Matrix Metalloproteinases (MMPs)  >>  MMP Colorimetric Substrate I

Product Name MMP Colorimetric Substrate I
Ac - PLG - SCH[CH2CH(CH3)2] - CO - LG - OC2H5
Size 1 mg
Catalog # 27096
US$ $121
Purity % Peak Area By HPLC ≥ 95%
Description

An exceptional MMP-2 (gelatinase A) and MMP-9 (gelatinase B) substrate this peptide is used for the continuous spectrophotometric assay of MMP-2 and MMP-9 in combination with a color-developing thiol-reactive agent, 4,4’-dithiodipyridine or Ellman’s Reagent (as indicators). This thiol peptide-enzyme reaction has a Km of 0.004 M and a kcat of 103 s-1. Using this substrate, collagenase can be detected at concentrations as low as 2 ng/mL. HPLC and tandem mass spectrometry are also used to analyze MMP-2 and MMP-9 in conjunction with this peptide substrate.

Storage -20°C
References Ref: Weingarten, H. and J. Feder, Anal. Biochem. 147, 437 (1985); Weingarten, H. et al. Biochem. 24, 6730 (1985).
Molecular Weight 655.9
Sequence
(One-Letter Code)
Ac-PLG-SCH[CH2CH(CH3)2]-CO-LG-OC2H5
Sequence
(Three-Letter Code)
Ac - Pro - Leu - Gly - SCH[CH2CH(CH3)2] - CO - Leu - Gly - OC2H5
     
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