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Assay-kits

SensoLyte® Homogeneous AFC Caspase-3/7 Assay Kit Fluorimetric - 1 kit

$372.00
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  • Cat.Number : AS-71114
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Central to the execution phase of apoptosis are the two closely related caspase-3 and caspase-7. They share common substrate specificity and structure, but differ completely in the sequence of their respective N-terminal regions. Both caspase-3 and caspase-7 have substrate selectivity for the amino acid sequence Asp-Glu-Val-Asp (DEVD).
The SensoLyte® Homogeneous AFC Capase-3/7 Assay Kit uses Ac-DEVD-AFC as the fluorogenic indicator for assaying caspase-3/7 activities. Upon caspase-3/7 cleavage, Ac-DEVD-AFC generates the AFC fluorophore which has bright blue fluorescence and can be detected at Ex/Em=380 nm/500 nm. A bi-function assay buffer in this kit is designed to lyze the cells and measure the enzyme activity at the same time. Thus, this kit can measure caspase-3/7 activity in cell culture directly in a 96-well or 384-well plate without a time-consuming cell extraction step. In case the cells are cultured in larger plates or flasks, a lysis buffer and protocol for cell lysate preparation are also conveniently included in the kit. The kit is suitable for high throughput screening of apoptosis inducers and inhibitors.

Specifications

Packaging
Kits components
  • Component A: Caspase-3/7 substrate, Ex/Em=380 nm/500 nm: 270 µL Component B: AFC, fluorescence reference standard, Ex/Em=380 nm/500 nm: 10 mM, 20 µL Component C: Caspase-3/7 inhibitor: 5 mM, 15 µL Component D: Assay Buffer: 30 mL Component E: DTT: 1 M, 1 mL Component F: 10X Lysis Buffer: 20 mL
Properties
Absorbance (nm)
  • 380
Emission (nm)
  • 500
Storage & stability
Storage Conditions
  • Store components A, B, C, and E at -20°C. Components D and F can be stored at 4°C for convenience. Component A and B protect from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34

Pharmacol Res . 2014 May 06 ; 85 6 | DOI : 10.1016/j.phrs.2014.04.013

  • C. Freise

Caspase-like activity during aging and cell death in the toxic dinoflagellate< i> Karenia brevis

Harmful Algae . 2013 Oct 20 ; 31 41 | DOI : 10.1016/j.hal.2013.08.005

  • J.G. Johnson

Glomerular cell death and inflammation with high-protein diet and diabetes

Nephrol Dial Transplant. . 2013 Jan 12 ; 28(7) 1711 | DOI : 10.1093/ndt/gfs579

  • R.L. Meek

The inhibitor of Ca(2+)-dependent K+ channels TRAM-34 blocks growth of hepatocellular carcinoma cells via downregulation of estrogen receptor alpha mRNA and nuclear factor-kappaB

Invest New Drugs . 2012 Oct 02 ; 31(2) 452 | DOI : 10.1007/s10637-012-9879-6

  • C. Freise

A hepatoprotective Lindera obtusiloba extract suppresses growth and attenuates insulin like growth factor-1 receptor signaling and NF-kappaB activity in human liver cancer cell lines

BMC Complement Altern Med . 2011 May 12 ; 11 39 | DOI : 10.1186/1472-6882-11-39

  • C. Freise

An evolved ribosome-inactivating protein targets and kills human melanoma cells in vitro and in vivo

Mol Cancer . 2010 Feb 03 ; 9 28 | DOI : 10.1186/1476-4598-9-28

  • M. Cheung

An active extract of Lindera obtusiloba inhibits adipogenesis via sustained Wnt signaling and exerts anti-inflammatory effects in the 3T3-L1 preadipocytes

J Nutr Biochem . 2010 Jan 25 ; 21(12) 1170 | DOI : 10.1016/j.jnutbio.2009.09.013

  • C. Freise

Extracts of Lindera obtusiloba induce antifibrotic effects in hepatic stellate cells via suppression of a TGF-β-mediated profibrotic gene expression pattern

J Nutr Biochem . 2008 Sep 27 ; 20(8) 597 | DOI : 10.1016/j.jnutbio.2008.06.003

  • M. Rueh

Synergistic inhibition of tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis in human pancreatic β cells by Bcl-2 and X-linked inhibitor of apoptosis

Hum Immunol . 2005 Mar 01 ; 66(3) 274 | DOI : 10.1016/j.humimm.2004.12.002

  • D. Ou