Login
Existing Account

Please login first to complete purchase/ quotation request, view custom order reports, or create favorites list.

Customer ID:
Password:
Stay Logged In


Forgot your Customer ID or Password?
New Account

Don't have an account with us yet? Please set up an account to place order or obtain customer services.

Peptides  >  Caspase Related Peptides  >>  Caspase 3 (Apopain) Substrate 1m, fluorogenic

Product Name Caspase 3 (Apopain) Substrate 1m, fluorogenic
Ac - DEVD - AMC
Size 25 mg
Catalog # 25262-25
US$ $605
Description

AMC (7-amino-4-methylcoumarin)-derived caspase substrates are widely used for the fluorimetric detection of various caspase activities. Cleavage of AMC peptides by caspases generates strongly fluorescent AMC that is monitored fluorimetrically at 440-460 nm with excitation of 340-350 nm.

Caspase-3 substrate with Km = 9.7 然; Caspase-7 substrate with Km = 11 然

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20蚓 desiccated and protected from light
References Koeplinger KA, et al. (2000). Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization of the active enzyme. Protein Expr Purif 18, 378-87; Garcia-Calvo M, et al. (1998). Inhibition of human caspases by peptide-based and macromolecular inhibitors. J Biol Chem 273, 32608-13; Thornberry NA, et al. (1997). A combinatorial approach defines specificities of members of the caspase family and granzyme B. Functional relationships established for key mediators of apoptosis. J Biol Chem 272, 17907-11; Rano TA, et al. (1997). A combinatorial approach for determining protease specificities: application to interleukin-1 beta converting enzyme (ICE). Chem Biol 4, 149-55; Margolin N, et al. (1997). Substrate and inhibitor specificity of interleukin-1 ?-converting enzyme and related caspases. J Biol Chem 272, 7223-8; Mizushima N, et al. (1996). Ceramide induces apoptosis via CPP32 activation. FEBS Lett 395, 267-71; Faubion WA, et al. (1999). Toxic bile salts induce rodent hepatocyte apoptosis via direct activation of Fas. J Clin Invest 103, 137-45.
Molecular Weight 675.5
Sequence
(One-Letter Code)
Ac-DEVD-AMC
Sequence
(Three-Letter Code)
Ac - Asp - Glu - Val - Asp - AMC
Product Citations Stoka, V. et al. (2006). Differential regulation of the intrinsic pathway of apoptosis in brain and liver during ageing. FEBS Lett 580, 3739.
Gukovsky, I. et al. (2003). Curcumin ameliorates ethanol and nonethanol experimental pancreatitis. AJP-GI 284, G85.
Lin-Shiau, SY. et al. (2003). Induction of apoptosis by rosemary polyphenols in HL-60 cells. Orient Foods Herbs chapter doi: 10.1021/bk-2003-0859.ch008.
Gukovskaya, AS. et al (2002). Cholecystokinin induces caspase activation and mitochondrial dysfunction in pancreatic acinar cells: Roles in cell injury processes of pancreatitis. J Biol Chem 277, 22595.
Yusef, K. et al. (2002). Trophoblast differentiation modulates the activity of caspases in primary cultures of term human trophoblasts. Ped Res 52, 411.
     
  < Back