A highly soluble fluorogenic MMP substrate for MMP-2, 8, 12, 13 and 14, containing the MMP cleavable Gly-Leu bond and EDANS/DABCYL. Fluorogenic assays using TNO211 are sensitive and can detect MMP activity in culture medium from endothelial cells and untreated synovial fluid from patients. Abs/Em = 340/490 nm.
Ref: Beekman, B. et al. FEBS Lett. 390, 221 (1996).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
DABCYL - (γ - Abu) - Pro - Gln - Gly - Leu - Glu(EDANS) - Ala - Lys - NH2