Caspases play important roles in apoptosis and cell signaling. They are also identified as drug-screening targets. AMC-based substrates yield blue fluorescence upon protease cleavage (Ex/Em=354 nm/442 nm). They are widely used to monitor caspase activity.
The SensoLyte® Caspase Profiling Kit contains a series of AMC-based peptide substrates as fluorogenic indicators for assaying caspase protease activities. The kit contains a well-designed plate in which a series of AMC-based caspase substrates are coated with both positive and negative controls. It provides the best solution for profiling caspases or caspase inhibitors.
The kit contains: • A 96-well plate coated with a series of AMC-based caspase substrates along with various controls* • Cell lysis buffer • Assay buffer • AMC (fluorescence reference standard for calibration) • A detailed protocol
*384-well plate format is available on custom basis.