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Host: Rabbit Species Reactivity: Human, mouse, rat Application: WB, IHC, ELISAProduct Description: Anti-beta-Actin (NT) rabbit polyclonal antibody is supplied as an epitope-affinity purified rabbit IgG 50 µg in 250 µl (0.2 mg/ml) of 1x PBS (pH 7.4) containing 0.05% sodium azide.Immunogen: Rabbit polyclonal anti-beta-actin (NT) was raised against a 21 amino acid peptide from near the N-terminus (NT) of human beta-actin (Genbank accession NP_001092.1).Application Notes: The following concentration ranges are recommended starting points. Optimal working concentration for specific application should be determined by the investigator.
ELISA for immunizing peptide: 1: 5,000-20,000Western blot: 1: 500-1,000