SensoLyte® Homogeneous AFC Caspase - 8 Assay Kit 200 assays (96 - well plate)
Caspase-8, also known as FLICE, MACH and Mch5, is a key enzyme in the apoptosis pathway. It is an upstream caspase that accepts signals from death receptors and initiates the apoptosis pathways. Caspase-8 cleaves inactive pro-forms of effector caspase-3, -6 and –7, thereby activating them. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis. The SensoLyte® Homogeneous AFC Caspase-8 Assay Kit uses Ac-IETD-AFC as the fluorogenic indicator to measure caspase-8 activity. Upon cleavage of the substrate by caspase-8, free AFC (7-amido-4-trifluoromethylcoumarin) emits a yellow-green fluorescence, which can be quantified at Ex/Em=380 nm/500 nm. A bi-functional assay buffer in this kit is optimized for cell lysis and measurement of the enzyme activity. This kit can assay caspase-8 activity in cells grown in a 96-well plate or in larger plates or flasks. The kit is adaptable for high throughput screening of apoptosis inducers and inhibitors.