This peptide is a C-terminal fragment of exendin-4. This 20-residue construct is over 95% folded in water at physiological pH, and exhibits a tightly folded tertiary structure in solution. Trp Cage is a highly stable mini-protein fold. It consists of a short helix, a 3,10 helix and a C-terminal poly-proline that packs against a Trp in the alpha helix. It is known to fold within 4 nanoseconds. This stable fast-folding Trp Cage sequence displays special stabilizing features specific to this miniprotein.
Ding, F. et al. Biophys. J.88, 147 (2005); Neidigh, J. et al. Nat. Struct. Biol.9, 425 (2002); Adams, C. et al. J. Am. Soc. Mass. Spectrom.15, 1087 (2004); Herman, R. et al. J. Biol. Chem.282, 9813 (2007).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Asn - Leu - Tyr - Ile - Gln - Trp - Leu - Lys - Asp - Gly -