Cross-linked Allophycocyanin (CL-APC), highly fluorescent stabilized phycobiliprotein, is chemically modified with SMCC. SMCC reacts with the primary amine on CL-APC and introduces maleimide groups to APC. These maleimide groups easily react with thiol groups of target protein without the need for any additional activation, resulting in convenient conjugation of APC with proteins. These conjugates are widely used in applications such as flow cytometry, live cell staining, and immunofluorescent staining.
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