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Antibodies  >  Primary Antibodies  >  Cytoskeleton  >>  Anti-Keratin-10 (IN), human Antibody polyclonal

Product Name Anti - Keratin - 10 (IN), human Antibody polyclonal
Size 50 g
Catalog # AS-55074
US$ $193

Host: Rabbit

Species Reactivity: Exclusive to human. Reactivity was validated by ELISA. Specificity was confirmed by Western blot in HT-29 and SCC-13 cell lysates.

Application: ELISA, WB

Product Description: Rabbit polyclonal Anti-Keratin-10 (IN) is supplied as an epitope affinity purified rabbit IgG, 50 g in 250 l (0.2 mg/l) of Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide.

Immunogen: Rabbit polyclonal Anti-Keratin-10 (IN) was raised against a synthetic peptide derived from the internal (IN) sequence of human keratin 10.

Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator.

ELISA for immunizing peptide: 1:5,000-20,000W
B: 1:500-2,000
IHC (recommended but not tested): 1:50-100
RELATED PRODUCT: An antibody raised against the C-terminus (CT) of Keratin-10, human is also available - Anti-Keratin-10 (CT), human Antibody, Rabbit Polyclonal (Cat# 55073)

Figure 1. Western blot of human Keratin-10 in HT-29 cell lysate (Lane A) and SCC-13 cell lysate (Lane B) labeled with the Anti-Keratin-10 (IN), human antibody (Cat# 55074) at 1:1000 dilution. An immunoreactive band at ~56 kDa was detected in both lysates. (Data kindly provided by Dr. Bishr Omary at University of Michigan).
Images Anti-Keratin-10 (IN), human Antibody <i>polyclonal</i>
Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Storage 4C
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