IMG-EN-AS-63899-01.jpg
Peptides

Fluorescein-Trp25-Exendin-4 (FLEX) - 0.5 mg

$372.00
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  • Cat.Number : AS-63899
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    In stock

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This peptide is Exendin-4 labeled with fluorescein at the Tryptophan residue. FLEX is equipotent to GLP-1(7-36)-amide and exendin-4 as an inhibitor of [125I] GLP-1 binding to the human GLP-1 receptor stably expressed in CHO cells, and maintains full biological potency and efficacy as measured by the stimulation of cAMP accumulation in these cells. FLEX binding to CHO/hGLP-1R membranes results in an increase in fluorescence anisotropy. The binding is specific and saturable (Kd = 2.0 +/- 0.4 nM), and GLP-1(7-36)-amide and Exendin-4 are equipotent inhibitors of FLEX binding to the human GLP-1 receptor. Thus, FLEX is a potent, biologically active ligand that is useful for the study of the binding and functional characteristics of the human GLP-1 receptor.

Specifications

Chemistry
Sequence one letter code
  • HGEGTFTSDLSKQMEEEAVRLFIE-(Trp-S-FAM)-LKNGGPSSGAPPPS-NH2
Sequence three letter code
  • H-His-Gly-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-(Trp-S-FAM)-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2
Molecular Mass/ Weight
  • 4606.4
Properties
Absorbance (nm)
  • 494
Emission (nm)
  • 521
Modification
Conjugation type
  • Fluorescent dyes
Modification Name
Conjugation
  • Conjugated
Quantity & Purity
Purity
  • Peak Area by HPLC ≥95%
Storage & stability
Form
  • Lyophilized
Storage Conditions
  • - 20 °C Protected from light
Activity
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Source / Species
  • Gila

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Citations

Chronic hyperglycemia downregulates GLP-1 receptor signaling in pancreatic β-cells via protein kinase A.

Mol Metabolism . 2015 Feb 03 ; 4(4) 265 | DOI : http://dx.doi.org/10.1016/j.molmet.2015.01.010.

  • S. Rajan

References

Fluorescein-Trp25-Exendin-4, a Biologically Active Fluorescent Probe for the Human GLP-1 Receptor

Peptides . 1996 Jan 28 ; 18(2) 319 | DOI : 10.1016/s0196-9781(96)00320-8

  • GG. Chicchi
  • et al