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Assay-kits

SensoLyte® Calcein Cell Viability Assay Kit Fluorimetric - 1 kit

$347.00
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  • Cat.Number : AS-72126
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    In production
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Calcein AM (calcein acetoxymethyl ester) is a non-fluorescent reagent that easily permeates cellular membrane. Once it permeates live cells, esterases remove the acetomethoxy group allowing calcein to bind to calcium, which results in a strong green fluorescence. The increase of fluorescence is proportional to the number of live cells. Inside dead cells, the non-fluorescent calcein AM remains intact due to the lack of active esterases. The fluorescent signal from the conversion of calcein AM to calcein can be monitored at Ex/Em=494nm /520 nm. The SensoLyte® Calcein Cell Viability Assay Kit provides a convenient tool to detect live cells. This kit can be used in proliferation, cytotoxicity, invasion, adhesion, migration and other cell-based assays.

Specifications

Packaging
Kits components
  • Component A: Calcein AM in anhydrous DMSO solution: 0.5 mM, 100 µL Component B: 10X Assay buffer: 100 mL
Properties
Absorbance (nm)
  • 494
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component A at -20°C, protect from light and moisture. Component B can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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Citations

Antiviral effect of strictinin on influenza virus replication

Antiviral Res . 2010 Jul 06 ; 88(1) 10 | DOI : 10.1016/j.antiviral.2010.06.008

  • R. Saha