Tissue-type plasminogen activator (tPA) is a serine protease that cleaves pro-enzyme plasminogen into active plasmin. It has been recognized that tPA play important roles in various physiological and pathological processes. In plasma, it functions as a thrombolytic enzyme and can be used for treatment of ischemic stroke. In the central nervous system, tPA promotes events associated with synaptic plasticity and cell death in a number of settings, such as cerebral ischemia and seizures. The SensoLyteŽ AMC tPA Activity Assay Kit is optimized for assaying tPA enzyme activities. This kit contains a fluorogenic substrate with a high reactivity and low background. tPA cleaves the substrate resulting in release of AMC (7-amino-4-methylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 354/442 nm. This kit can be used for high throughput screening of tPA inducers and inhibitors.