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Assay Kits  >  Renin Assay Kits  >>  SensoLyte® 520 Mouse Renin Assay Kit *Fluorimetric*

Product Name SensoLyte® 520 Mouse Renin Assay Kit *Fluorimetric*
Size 1 kit
Catalog # 72161
US$ $599
Description

Renin, a highly specific aspartyl protease, cleaves angiotensinogen, to yield angiotensin I. Angiotensin I is further converted into angiotensin II by ACE (Angiotensin Converting Enzyme). Angiotensin II constricts blood vessels leading to increased blood pressure. Discovery of new renin inhibitors for treatment of hypertension is a promising area of research. The SensoLyte® 520 Mouse Renin Assay Kit provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of mouse renin activity using a 5-FAM/QXL™520 FRET peptide. In the FRET peptide the fluorescence of 5-FAM is quenched by QXL™520. Upon cleavage into two separate fragments by renin, the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission = 490/520 nm. The kit contains: 5-FAM/QXL™520-based FRET mouse specific renin substrate (Ex/Em=490/520 nm upon cleavage), assay buffer, mouse recombinant prorenin, reagents for conversion of prorenin to renin, inhibitor, fluorescence reference standard for calibration, and an optimized assay protocol.

100 assays (96-well plate)

Detailed Information Datasheet
Material Safety Data Sheets (MSDS)
Product Citations Kong, J. et al. (2013). VDR Attenuates Acute Lung Injury by Blocking Ang-2-Tie-2 Pathway and Renin-Angiotensin System. Mol Endocrinol 27, 2116. doi: 10.1210/me.2013-1146.
Kakoki, M. et al. (2013). Primary aldosteronism and impaired natriuresis in mice underexpressing TGFβ1. PNAS 110, 5600. doi: 10.1073/pnas.1302641110.
Takada, S. et al. (2013). Angiotensin II receptor blocker improves the lowered exercise capacity and impaired mitochondrial function of the skeletal muscle in type 2 diabetic mice. J Appl Physiol 114, 844. doi: 10.​1152/​japplphysiol.​00053.​2012.
Prysyazhna, O. et al. (2012). Single atom substitution in mouse protein kinase G eliminates oxidant sensing to cause hypertension. Nature Med 18, 286.
Tsukuda, K. et al. (2012). Influence of angiotensin II type 1 receptor-associated protein on prenatal development and adult hypertension after maternal dietary protein restriction during pregnancy. J Am Soc Hypertension 6, 324. doi:10.1016/j.jash.2012.07.001
Wang, Y. et al. (2012). Vitamin D receptor signaling in podocytes protects against diabetic nephropathy. J Am Soc Nephrol doi:10.1681/ASN.2012040383.
     
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