The SensoLyte® Rh110 Elastase Assay Kit detects elastase activity using a fluorogenic peptide substrate. Upon elastase cleavage, the peptide releases the Rh110 fluorophore with bright green fluorescence that can be detected at Ex/Em=496 /520 nm. The increase in fluorescence intensity is directly proportional to enzyme activity. The kit does not require any separation steps and can be used to continuously measure the kinetics of elastase. The longer-wavelength spectra and higher extinction coefficient of the Rh110 provide greater sensitivity and less interference from screening compounds.
The kit contains: • Rh110 labeled fluorogenic peptide substrate (Ex/Em=490/520 nm upon cleavage) • Fluorescence reference standard for calibration • Elastase enzyme (as positive control) • Assay buffer • Elastase inhibitor • Detailed protocol