Human Sirtuin 2, recombinant

Histone deacetylases (HDACs) act as transcriptional repressors of genes catalyzing the removal of acetyl groups from a ε-N-acetyl lysine of histone. Sirtuin 2 (SIRT2) belongs to a unique class of nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases (class III HDACs) that target multiple protein substrates to execute diverse biological functions. Sirtuin 2 is the member of the family of Sir2 (Silent Information Regulator 2) proteins. Besides histones, substrates for SIRT2 also include various transcription factors, the tumor suppressor p53 and alpha-tubulin. Human SIRT2 is a cytoplasmic protein that increases in abundance during mitosis and regulates major events of cytokinesis.

The recombinant human Sirtuin 2 (GenBank Accession #: NM_030593) with 13-319 amino acids and His tag at its C-terminal was expressed in E. coli. The molecular mass of the enzyme is approximately 35.5 kDa on SDS-PAGE. Its activity can be measured using enzymatic assay with a fluorogenic or FRET substrate

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