This peptide is histone H3 (1-21) with symmetrical dimethylation at Arg2 and acetylation at Lys4, followed by a C-terminal GGK with biotinylation on the side chain of Lys. Dimethylation of the guanidinium group of Arg2 is associated with transcriptional silencing. Lys4 acetylation acts as a chromodomain switch by weakening the interaction between Chp1 and the H3K9 methylated tail, thus facilitating binding of HP1 proteins. Provided at >95% peptide purity, this peptide was dissolved in distilled water at 1 mg/ml and re-lyophilized to powder form.
Xhemalce, B. and T. Kouzarides Gene Dev24, 647 (2010); Musselman, CA and TG. Kutateladze Mol Interv9, 314 (2010); Kirmizis, A. et al. Nat Struct Mol Biol16, 449 (2009); Garcia, B. et al. J Biol Chem282, 7641 (2007).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ala - Arg(Me2s) - Thr - Lys(Ac) - Gln - Thr - Ala - Arg - Lys - Ser - Thr - Gly - Gly - Lys - Ala - Pro - Arg - Lys - Gln - Leu - Ala - Gly - Gly - Lys(Biotin) - OH