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Assay-kits

SensoLyte® NADP/NADPH Assay Kit Fluorimetric - 1 kit

$554.00
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  • Cat.Number : AS-72206
  • Availability :
    In production
  • Shipping conditions : Ice fees will apply

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Nicotinamide nucleotides are key players in the energy transforming and oxidation-reduction reactions of a cell. NADP and NADPH act as allosteric effectors in modulating enzyme activities. The reducing power of NADPH is a required cofactor for enzymes that are pro-oxidants, such as nitric oxide synthase and NADPH oxidase. NADP/NADPH ratio is involved in the control of the oxidative shunt. Each of these forms has a particular role during differentiation and similarity processes as well as being target functions in the cytotoxic action of ionizing radiations and alkylating agents. The SensoLyte® NADP/NADPH Assay Kit utilizes an optimized enzymatic recycling method for the quantification of NADP/NADPH without interfering with NAD/NADH. NADP is converted to NADPH in this enzyme-cycling reaction. The kit contains a fluorogenic reagent, producing fluorescence when reacted with NADPH. The resulting red fluorescence can be monitored at excitation /emission= 560/590 nm. The intensity of the fluorescence produced is proportional to NADP/NADPH concentration.

Specifications

Packaging
Kits components
  • Component A: Reagent A: 4 mL Component B: Reagent B: 1 mL Component C: Enzyme Cycling Mix: 70 µL Component D: Assay Buffer: 50 mL Component E: NADP Standard: 100 µM, 20 µL Component F: NADP Extraction Buffer: 25 mL Component G: NADPH Extraction Buffer: 25 mL
Properties
Absorbance (nm)
  • 560
Emission (nm)
  • 590
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store components A, B and E at -20°C and protect from light. Components D, F and G can be stored at room temperature for convenience.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 1.5 nM
Research Area
Sub-category Research Area
Usage
  • Research use

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