This kit is optimized to detect anti-human MOG (1-125) IgG in rat serum or cerebrospinal fluid. Wells are pre-coated with recombinant human MOG (1-125) protein and pre-blocked with BSA. The amount of anti-human MOG IgG in serum or cerebrospinal fluid is quantified using ELISA. Rat anti-Human MOG IgG standard is included. Ample materials and reagents are provided to perform 96 assays in a 96-well plate format.
o Pre-coated and pre-blocked 96-well strip plate o Ready-to-use substrate solution and other assay components o 2-3 hours assay time at room temperature
Minimal Sample Size
o Requires only 0.5-1 μl of serum or cerebrospinal fluid to perform assay
o Detects as low as 1 ng/ml anti-human MOG (1-125) IgG
Broad Dynamic Range
o 8-500 ng antibody/ml serum (depending on colorimetric developing time)