This is a Histone 3 peptide trimethylated at lysine 36. It's role has been indicative of defining exons, as certain nucleosome-enriched exons are observed to be enriched in some histone modifications. It is belived that this pattern of modification as seen with this H3K36me3 peptide, influences alternative splicing, perhaps by signaling effector proteins to mark particular exons for inclusion in the final transcript as they exit the RNA Polymerase II complex. It has also been shown in yeast that H3K36me3 gets deposited on histones as they are displaced by RNA polymerase II (RNAPII) during transcription. H3K36me3 then serves as a mark for HDACs to bind and deacetylate the histones.
This peptide is Histone H3 amino acid residues 21 to 44 tri-methylated at Lys-36 with an additional C-terminal glycine followed by a biotinylated lysine. Provided at >95% peptide purity, this peptide was dissolved in distilled water at 1 mg/ml and re-lyophilized to powder form.
1, Luco RF. et al. Science, 327:996-1000 (2010) 2, Carrozza MJ. et al. Cell, 123:581-592 (2005).
Sequence (One-Letter Code)
Sequence (Three-Letter Code)
H - Ala - Thr - Lys - Ala - Ala - Arg - Lys - Ser - Ala - Pro - Ala - Thr - Gly - Gly - Val - Lys(Me3) - Lys - Pro - His - Arg - Tyr - Arg - Pro - Gly - Gly - Lys(Biotin) - OH