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Assay-kits

SensoLyte® 520 Cathepsin L Assay Kit Fluorimetric - 1 kit

$582.00
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  • Cat.Number : AS-72218-
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Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin L, a lysosomal endopeptidase, is a member of the papain-like family of cysteine proteinases. It is involved in the promotion of tumor cell invasion, antigen processing and turnover of intracellular and secreted proteins.
The SensoLyte® 520 Cathepsin L Activity Assay Kit uses a QXL™ 520/HiLyte™ Fluor 488 labeled FRET peptide substrate for measuring enzyme activity. The long wavelength FRET substrate is designed based on a sequence surrounding the cleavage site of Cathepsin L. The QXL™ 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin L cleaves the FRET substrate, it results in an increase of HiLyte Fluor™ 488 fluorescence and is monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, the optimal pH for Cathepsin L activity.

Specifications

Packaging
Kits components
  • Component A: QXL™ 520/HiLyte™ Fluor 488, Cathepsin L substrate, Ex/Em=490 nm/520 nm upon cleavage: 1 mM, 50 µL Component B: HiLyte Fluor™ 488, fluorescence reference standard, Ex/Em=490 nm/520 nm: 1 mM, 10 µL Component C: Cathepsin L, human recombinant: 0.1 mg/mL, 20 µL Component D: Assay Buffer: 20 mL Component E: Cathepsin L inhibitor: 100 µM, 10 µL Component F: DTT: 1 M, 200 µL
Chemistry
UniProt number
  • P07711
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

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Citations

Dual Inhibition of HIV-1 and Cathepsin L Proteases by Sarcandra glabra

Molecules . 2022 Sep 01 ; 27(17) 5552 | DOI : 10.3390/molecules27175552

  • B. Pan
  • et al

Novel dithiocarbamates selectively inhibit 3CL protease of SARS-CoV-2 and other coronaviruses

Eurn J Med Chem . 2023 Mar 15 ; 250 115186 | DOI : https://doi.org/10.1016/j.ejmech.2023.115186

  • L. Brier
  • et al