Anti - Keratin - 10 (IN), human Antibody polyclonal
Host: Rabbit Species Reactivity: Exclusive to human. Reactivity was validated by ELISA. Specificity was confirmed by Western blot in HT-29 and SCC-13 cell lysates.Application: ELISA, WBProduct Description: Rabbit polyclonal Anti-Keratin-10 (IN) is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 mg/µl) of Phosphate Buffered Saline (pH 7.4) containing 0.05% Sodium Azide. Immunogen: Rabbit polyclonal Anti-Keratin-10 (IN) was raised against a synthetic peptide derived from the internal (IN) sequence of human keratin 10.Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator. ELISA for immunizing peptide: 1:5,000-20,000WB: 1:500-2,000 IHC (recommended but not tested): 1:50-100 RELATED PRODUCT: An antibody raised against the C-terminus (CT) of Keratin-10, human is available - Anti-Keratin-10 (CT), human Antibody, Rabbit PolyclonalFigure 1. Western blot of human Keratin-10 in HT-29 cell lysate (Lane A) and SCC-13 cell lysate (Lane B) labeled with the Anti-Keratin-10 (IN), human antibody (Cat# 55074) at 1:1000 dilution. An immunoreactive band at ~56 kDa was detected in both lysates. (Data kindly provided by Dr. Bishr Omary at University of Michigan).