Host: Rabbit Species Reactivity: Human; Predicted by homology: mouse, rat, bovine, dog, hamster, and rabbit; Others not tested.Application: IHCProduct Description: Rabbit polyclonal Anti-Nestin antibody is supplied as 0.25 ml of affinity purified rabbit IgG at 200 µg/ml in 1X PBS (pH 7.4) with 0.05% sodium azide. Immunogen: Rabbit polyclonal Anti-Nestin antibody was raised against a synthetic peptide derived from internal region of human nestin protein.Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator. IHC (Formalin/paraffin): 1:200 for 30 min at room temperature (Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min). Figure 1. IHC of human liver carcinoma cells (formalin-fixed/ paraffin-embedded) stained with rabbit polyclonal Anti-Nestin antibody (Cat# 55935).