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Assay-kits

SensoLyte® 520 Cathepsin E Assay Kit Fluorimetric - 1 kit

$582.00
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  • Cat.Number : AS-72222
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Cathepsin E is a non-lysosomal aspartic proteinase of the pepsin superfamily. It plays an important role in the protein degradation, the generation of bioactive proteins, and antigen processing. Recent studies have particularly suggested that Cathepsin E is important in host defense against cancer cells and invading microorganisms.
The SensoLyte® 520 Cathepsin E Activity Assay Kit is a homogeneous assay that can be used to detect the enzyme activity in biological samples or in purified enzyme preparations. The unique long wavelength FRET substrate employed in the assay was designed to reduce the cross reactivity of Cathepsin D. A QXL™ 520/HiLyte™ Fluor 488 pair is used for optimal quenching of the intact substrate. When active Cathepsin E cleaves the FRET substrate, it results in an increase of HiLyte™ Fluor 488 fluorescence, monitored at excitation/emission = 490 nm/520 nm. The fluorescent signal from HiLyte™ Fluor 488 is stable at low pH, an optimal pH for cathepsin activity. This assay can detect as low as 2 ng/mL active Cathepsin E.

Specifications

Packaging
Kits components
  • Component A: QXL™ 520/HiLyte™ Fluor 488, Cathepsin E substrate, Ex/Em=490 nm/520 nm upon cleavage: 2 mM, 50 µL Component B: HiLyte™ Fluor 488, fluorescence reference standard, Ex/Em=490 nm/520 nm: 2 mM, 10 µL Component C: Human recombinant Cathepsin E: 0.1 mg/mL, 10 µL Component D: Assay Buffer: 20 mL Component E: Pepstatin A: 10 µM, 20 µL
Chemistry
UniProt number
  • P14091
Properties
Absorbance (nm)
  • 490
Emission (nm)
  • 520
Storage & stability
Storage Conditions
  • Store component C at -80°C. Store all other components at -20°C. Component D can be stored at room temperature for convenience. Protect components A and B from light and moisture.
Activity
Application
Biomarker Target
Detection Method
Detection Limit
  • 2 ng/ml
Research Area
Sub-category Research Area
Usage
  • Research use
Source
Host

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