Host: Rabbit Species Reactivity: Human, mouse, rat and dog; others not tested. Application: IHCProduct Description: Rabbit polyclonal Anti-Calpain (IN) Antibody is supplied as 0.25 ml of affinity purified rabbit IgG at 200 µg/ml in 1X PBS (pH 7.4) with 0.05% sodium azide. Immunogen: Rabbit polyclonal Anti-Calpain Antibody was raised against a synthetic peptide derived from internal (IN) region of human calpain protein. Application Notes: The following concentration ranges are recommended starting points. Optimal working concentrations for specific application should be determined by the investigator. IHC: 1:100 for 30 min at room temperature. (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temperature for 20 min). Figure 1. IHC of human breast carcinoma cells (formalin-fixed/paraffin-embedded) stained with rabbit polyclonal Anti-Calpain (IN) Antibody (Cat# 55938).