Species Reactivity: Species reactivity includes human, mouse, rat, bovine and monkey, while others have not been tested. This antibody was evaluated for specificity with dot blot assay using synthetic tau peptides.
Application: ELISA, dot blot, WB
Product Description: Rabbit polyclonal Anti-Tau Antibody (Acetyl K280) is supplied as an epitope affinity purified rabbit IgG, 50 µg in 250 µl (0.2 µg/µl) 1x PBS (pH 7.5) containing BSA and 0.02% Sodium Azide.
Immunogen: Rabbit Anti-Tau (Acetyl K280) polyclonal antibody was raised against a synthetic peptide corresponding to human Tau sequence surrounding Lysine 280.
Application Notes: The following concentration ranges are recommended ranges. Optimal working concentrations for specific application should be determined by the investigator.
Dot Blot: 1:200-2,000
Immunoprecipitation*: 3-5 µg/extract from 107 cells
*Recommended but not tested
Anti-Tau Antibody (K280), Rabbit Polyclonal - Antibody raised against Tau K280. It recognizes both the acetylated and non-acetylated Tau K280
Anti-Tau Antibody (GlcNAc Ser400), Rabbit Polyclonal - Antibody raised against Tau glycosylated Ser400
Anti-Tau Antibody (Ser400), Rabbit Polyclonal - Antibody raised against Tau Ser400. This antibody recognizes Ser400 modifications, including glycosylated and phosphorylated Ser400
Anti-Tau Antibodies - Wide selection of phospho & non-phospho specific 36+ Tau antibodies
Tau (441) Human Recombinant Proteins, with or without GST tag
Tau Peptides - 6 isoforms &[Ser(OGlcNAc)]400-KWK-Tau (388-411)-KK(Biotin)-NH2
Spotlight: Tau Products - Antibodies, Recombinant Proteins and Peptides
Figure 1. Dot blot of rabbit anti-Tau (Acetyl K280) polyclonal antibody using synthetic peptides: Tau (Acetyl K280), Tau (K280) dotted at 0.02, 0.2 and 1 µg.
Figure 2. WB of recombinant Tau proteins using rabbit anti-Tau (Acetyl K280) polyclonal antibody. M: Marker. Lane 1: Recombinant Tau protein acetylated by p300 enzyme. Lane 2: Recombinant Tau protein.