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Labeling-detection

CyLyte Fluor 5, NHS ester

$124.00
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  • Cat.Number : AS-89357-1
  • Availability :
    In production
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CyLyte Fluor 5 NHS ester dye has the same organic dye structure as that of Cy®5 NHS ester dye. However, the counter-ion used in the final formulation of AnaSpec’s CyLyte Fluor 5 NHS ester dye does vary compared to the Cy®5 NHS ester dye. This CyLyte Fluor 5 NHS ester is a reactive dye used for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This highly sensitive fluorophore has its emission maximum in the red region exhibiting maximum sensitivity thus minimizing background interference from biological specimen. [Cy®5 is a registered trademark of GE Healthcare]. Abs/Em= 648/663 nm; ε= 250,000 M-1cm-1 in PBS buffer (pH7.4)

Specifications

Chemistry
CAS registry number
  • 146368-14-1
Molecular Formula
  • C37H43N3O10S2
Molecular Mass/ Weight
  • 753.9
Properties
Absorbance (nm)
  • 648
Emission (nm)
  • 663
Color
Quantity & Purity
Purity
  • Peak Area by HPLC ≥90%
Storage & stability
Form
  • Lyophilized
Resuspension condition
  • DMF or DMSO: Prepare 10 mg/ml dye stock solution in DMF or DMSO. Completely dissolve all dye content by vortexing. Dye solution should be prepared fresh, immediately before an experiment. Long-term storage in solution form may reduce dye activity. Any solution containing the dye should be protected from light.
Storage Conditions
  • -20°C Protected from Light
Activity
Application
Detection Method
Research Area
Sub-category Research Area
Usage
  • Research use

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