MOG protein, with sequences corresponding to human, mouse and rat extracellular domain, together with a 6x His tag were expressed in E. coli
and purified from urea denatured bacterial lysate. The molecular weight found in all 3 recombinant proteins was 14.23 kDa. These proteins were shown to induce EAE in either mouse (for the human and mouse rMOGs) or rat (for rat rMOG).
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