PTM Lysines & Arginines


Proteins undergo post-translational modifications (PTM) as a mechanism to expand the coding capacity of genes.1 PTMs found in histone "tails" (amino termini) such as acetylation, methylation and phosphorylation affect chromatin structure and exquisitely regulate gene expression.2-7

The use of synthetic histone peptides containing PTMs has helped unraveled some of the mysteries of PTMs and their interaction with downstream effectors. For example, the use of 15-20 amino acid long histone peptides containing biotin either on the N or C-terminus used in pull-down assays has advanced the understanding of these epigenetic relationships.8-9

At AnaSpec, EGT Group, we are pleased to be a source of premium quality amino acids, whether Fmoc or Boc protected, for PTM peptide synthesis. In addition to PTM lysines and arginines, Fmoc protected phosphorylated D and L-serines and threonines are also available.

Request PTM peptide synthesis price quotation.

Acetylated Lysines

Product

Size

Catalog #

Fmoc-Lys(Ac)-OH
N-α-Fmoc-N-ε-acetyl-L-lysine,
CAS# 159766-56-0

Fmoc-Lys(Ac)-OHN-a-Fmoc-N-e-acetyl-L-lysine, CAS# 159766-56-0 1 g

5 g

22502

23356

Boc-Lys(Ac)-OH
N-α-t-Boc-N-ε-acetyl-L-lysine,
CAS# 6404-26-8

Boc-Lys(Ac)-OHN-a-t-Boc-N-e-acetyl-L-lysine,CAS# 6404-26-8

1 g

5 g

23084

23085


Methylated (mono, di & tri) Lysines

Product

Size

Catalog #

Fmoc-Lys(Me, Boc)-OH
N-α-Fmoc-N-ε,ε-t-Boc-methyl-L-lysine

Fmoc-Lys(Me, Boc)-OHN-a-Fmoc-N-e,e-t-Boc-methyl-L-lysine

0.25 g

1 g

5 g

60327-025

60327-1

60327-5

Fmoc-Lys(Me)2-OH • HCl
Fmoc-Lys(Me)2-OH chloride; Fmoc-Lys(Me)2–OH

CAS# 252049-10-8

Fmoc-Lys(Me)2-OH • HClFmoc-Lys(Me)2-OH chloride; Fmoc-Lys(Me)2–OHCAS# 252049-10-8

250 mg

1 g

28211

28212

Boc-Lys(Me)2-OH
Boc-Nε,ε -dimethyl-L-lysine

Boc-Lys(Me)2-OHBoc-Ne,e -dimethyl-L-lysine

250 mg

1 g

60025-025

60025-1

Fmoc-Lys(Me3)-OH chloride

CAS# 201004-29-7

Fmoc-Lys(Me3)-OH chlorideCAS# 201004-29-7

250 mg

500 mg

1 g

53099-F025

53099-F05

53099-F1


Methylated (mono & di- symmetrical & asymmetrical) Arginines

Product

Size

Catalog #

Fmoc-Arg(Me)(Pbf)–OH

CAS# 135616-49-7

Fmoc-Arg(Me)(Pbf)–OHCAS# 135616-49-7

0.25 g

1 g

65281-025

65281-1

Fmoc-Arg(Me)2-OH (asymmetrical)
Fmoc-Nω,ω-dimethyl-L-arginine (asymmetrical)

CAS# 268564-10-9

Fmoc-Arg(Me)2-OH (asymmetrical)Fmoc-N?,?-dimethyl-L-arginine (asymmetrical)CAS# 268564-10-9

250 mg

1 g

26218-F025

26218-F1

Fmoc-Arg(Me)2-OH (symmetrical)
Fmoc-Nω,ω-dimethyl-L-arginine (symmetrical)

Fmoc-Arg(Me)2-OH (symmetrical)Fmoc-N?,?-dimethyl-L-arginine (symmetrical)

250 mg

1 g

26217-F025

26217-F1

Deiminated (citrullinated) Arginines

Product

Size

Catalog #

Fmoc-DL-citrulline

Fmoc-DL-citrulline

1 g

5 g

26216-F1

26216-F5

Boc-DL-citrulline

Boc-DL-citrulline

1 g

5 g

26216-B1

26216-B5

Fmoc-L–citrulline

CAS# 133174-15-9

Fmoc-L–citrullineCAS# 133174-15-9

1 g

5 g

21359

21361

Boc-L–citrulline

CAS# 45234-13-7

Boc-L–citrullineCAS# 45234-13-7

1 g

5 g

26215-B1

26215-B5

Phosphorylated Serines

Product

Size

Catalog #

Fmoc-Ser[PO(OBzl)-OH]-OH
N-α-Fmoc-O-benzyl-L–phosphoserine
CAS# 158171-14-3

Fmoc-Ser[PO(OBzl)-OH]-OHN-a-Fmoc-O-benzyl-L–phosphoserineCAS# 158171 - 14 - 3

1 g

5 g

25 g

50 g

100 g

1 cartridge

10 cartridges

20419

20420

20420-25

20420-50

20420-100

29869

23219

Fmoc-D-Ser[PO(OBzl)-OH]-OH
N-α-Fmoc-O-benzyl-D-phosphoserine

Fmoc-D-Ser[PO(OBzl)-OH]-OHN-a-Fmoc-O-benzyl-D-phosphoserine

1 g

5 g

60083-1

60083-5

Phosphorylated Threonines

Product

Size

Catalog #

Fmoc-Thr[PO(OBzl)-OH]-OH
N-α-Fmoc-O-benzyl-L-phosphothreonine CAS# 175291-56-2

Fmoc-Thr[PO(OBzl)-OH]-OHN-a-Fmoc-O-benzyl-L-phosphothreonine       CAS# 175291-56-2

1 g

5 g

25 g

50 g

100 g

20835

20836

20836-25

20836-50

20836-100

Fmoc-D-Thr[PO(OBzl)-OH]-OH
N-α-Fmoc-O-benzyl-D-phosphothreonine

Fmoc-D-Thr[PO(OBzl)-OH]-OHN-a-Fmoc-O-benzyl-D-phosphothreonine

1 g

5 g

60557-1

60557-5

Phosphorylated D-Tyrosine

Fmoc-D- Tyr[PO(OBzl)OH] -OH
N-α-Fmoc-O-benzyl-D-phosphotyrosine

Fmoc-D-Tyr[PO(OBzl)OH]-OH

1 g

5 g

61428-1

61428-5

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Reference:

1. Walsh, CT. et al. Angew. Chem. Int. Ed. 44, 7342 (2005).

2. Grunstein, M. et al. J. Cell Sci. Suppl. 19, 29 (1995).

3. Strahl, BR and CD Allis, Nature 403, 41 (2000).

4. Strahl, BD. et al. Proc. Natl. Acad. Sci. 96, 14967 (1999).

5. Jenuwein, T. and CD. Allis Science 293, 1074 (2001).

6. Sterner, DE. et al. Microbiol. Mol. Biol. Rev. 64, 435 (2000).

7. Lachner, M. et al. J. Cell Sci. 116, 2117 (2003).

8. Protocol Online http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=4089.

9. Wysocka, J. Methods 40, 339 (2006).