Histone PTM Peptide Modifications


Proteins undergo post-translational modifications (PTM) as a mechanism to expand the coding capacity of genes.1 PTMs found in histone "tails" (amino termini) such as acetylation, methylation and phosphorylation affect chromatin structure and exquisitely regulate gene expression.2-7

The use of synthetic histone peptides containing PTMs has helped unraveled some of the mysteries of PTMs and their interaction with downstream effectors. For example, the use of 15-20 amino acid long histone peptides containing biotin either on the N or C-terminus used in pull-down assays has advanced the understanding of these epigenetic relationships.8-9

At AnaSpec, EGT Group, we are pleased to be a source of premium quality amino acids, whether Fmoc or Boc protected, for PTM peptide synthesis. In addition to PTM lysines and arginines, Fmoc protected phosphorylated D and L-serines and threonines are also available.

  • PTM Amino Acids - methylated, acetylated, citrullinated, phosphorylated, glycosylated
  • Histone Peptides (catalog & custom)
  • Custom Substrate Libraries

Request PTM peptide synthesis price quotation.

Acetylated Lysines

Product

Size

Catalog #

Fmoc-Lys(Ac)-OH
N-α-Fmoc-N-ε-acetyl-L-lysine,
CAS# 159766-56-0

Fmoc-Lys(Ac)-OHN-a-Fmoc-N-e-acetyl-L-lysine, CAS# 159766-56-0 1 g

5 g

AS-22502

AS-23356


Methylated (mono, di & tri) Lysines

Product

Size

Catalog #

Fmoc-Lys(Me, Boc)-OH
N-α-Fmoc-N-ε,ε-t-Boc-methyl-L-lysine

Fmoc-Lys(Me, Boc)-OHN-a-Fmoc-N-e,e-t-Boc-methyl-L-lysine

0.25 g

1 g

AS-60327-025

AS-60327-1

Fmoc-Lys(Me)2-OH • HCl
Fmoc-Lys(Me)2-OH chloride; Fmoc-Lys(Me)2–OH

CAS# 252049-10-8

Fmoc-Lys(Me)2-OH • HClFmoc-Lys(Me)2-OH chloride; Fmoc-Lys(Me)2–OHCAS# 252049-10-8

250 mg

1 g

AS-28211

AS-28212

Fmoc-Lys(Me3)-OH chloride

CAS# 201004-29-7

Fmoc-Lys(Me3)-OH chlorideCAS# 201004-29-7

250 mg

1 g

AS-53099-F025

AS-53099-F1


Methylated (mono & di- symmetrical & asymmetrical) Arginines

Product

Size

Catalog #

Fmoc-Arg(Me)(Pbf)–OH

CAS# 135616-49-7

Fmoc-Arg(Me)(Pbf)–OHCAS# 135616-49-7

0.25 g

1 g

AS-65281-025

AS-65281-1

Fmoc-Arg(Me)2-OH (asymmetrical)
Fmoc-Nω,ω-dimethyl-L-arginine (asymmetrical)

CAS# 268564-10-9

Fmoc-Arg(Me)2-OH (asymmetrical)Fmoc-N?,?-dimethyl-L-arginine (asymmetrical)CAS# 268564-10-9

250 mg

1 g

AS-26218-F025

AS-26218-F1

Fmoc-Arg(Me)2-OH (symmetrical)
Fmoc-Nω,ω-dimethyl-L-arginine (symmetrical)

Fmoc-Arg(Me)2-OH (symmetrical)Fmoc-N?,?-dimethyl-L-arginine (symmetrical)

250 mg

1 g

AS-26217-F025

AS-26217-F1

Deiminated (citrullinated) Arginines

Product

Size

Catalog #

Fmoc-L–citrulline

CAS# 133174-15-9

Fmoc-L–citrullineCAS# 133174-15-9

5 g

AS-21361

Phosphorylated Serines

Product

Size

Catalog #

Fmoc-Ser[PO(OBzl)-OH]-OH
N-α-Fmoc-O-benzyl-L–phosphoserine
CAS# 158171-14-3

Fmoc-Ser[PO(OBzl)-OH]-OHN-a-Fmoc-O-benzyl-L–phosphoserineCAS# 158171 - 14 - 3

1 g

5 g

AS-20419

AS-20420

Phosphorylated Threonines

Product

Size

Catalog #

Fmoc-Thr[PO(OBzl)-OH]-OH
N-α-Fmoc-O-benzyl-L-phosphothreonine CAS# 175291-56-2

Fmoc-Thr[PO(OBzl)-OH]-OHN-a-Fmoc-O-benzyl-L-phosphothreonine       CAS# 175291-56-2

1 g

5 g

AS-20835

AS-20836

Fmoc-D-Thr[PO(OBzl)-OH]-OH
N-α-Fmoc-O-benzyl-D-phosphothreonine

Fmoc-D-Thr[PO(OBzl)-OH]-OHN-a-Fmoc-O-benzyl-D-phosphothreonine

1 g

AS-60557-1

Phosphorylated D-Tyrosine

Fmoc-D- Tyr[PO(OBzl)OH] -OH
N-α-Fmoc-O-benzyl-D-phosphotyrosine

Fmoc-D-Tyr[PO(OBzl)-OH]-OHN-a-Fmoc-O-benzyl-D-phosphotyrosine

1 g

AS-61428-1

Related Products:

Histone H1 Peptides - Full Listing

Histone H2A Peptides - Full Listing

Histone H2B Peptides - Full Listing

Histone H3 Peptides – Full Listing

Histone H4 Peptides - Full Listing

Histone Related Substrates – Full Listing
SensoLyte® 520 HDAC Assay Kit

SensoLyte® LSD1 Assay Kit

SensoLyte® 520 Deubiquitination Assay Kit
SensoLyte® 440 Deubiquitination Assay Kit
SensoLyte® 520 FRET SIRT1 Assay Kit
SensoLyte® Green SIRT1 Assay Kit
SensoLyte® 520 FRET SIRT2 Assay Kit
SensoLyte® Green SIRT2 Assay Kit
SensoLyte® HAT (p300) Assay Kit
Monoclonal Antibody against 5-Methylcytidine

Reference:

1. Walsh, CT. et al. Angew. Chem. Int. Ed. 44, 7342 (2005).

2. Grunstein, M. et al. J. Cell Sci. Suppl. 19, 29 (1995).

3. Strahl, BR and CD Allis, Nature 403, 41 (2000).

4. Strahl, BD. et al. Proc. Natl. Acad. Sci. 96, 14967 (1999).

5. Jenuwein, T. and CD. Allis Science 293, 1074 (2001).

6. Sterner, DE. et al. Microbiol. Mol. Biol. Rev. 64, 435 (2000).

7. Lachner, M. et al. J. Cell Sci. 116, 2117 (2003).

8. Protocol Online http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=4089.

9. Wysocka, J. Methods 40, 339 (2006).