Protease Activity Assay Kits
Protease Assay kits

AnaSpec, EGT Group is pleased to spotlight our wide portfolio of iD SensoLyteŽ Protease Activity Assay kits for the study of proteases having roles in specific diseases. Further, our Custom iD Optimization services caters to custom assay development with high quality and minimal lead time.

Catalog & Custom Protease Activity Assays- Features & Benefits
  • Ready-to-use mix and read homogeneous format ideal for HTS.
  • All essential components for performing assays in 96-well plate formats.
  • Detailed protocols and references included, ensuring value and reliability.
  • Proprietary substrates (FRET pairs) in many of our protease kits.

  • SensoLyteŽ Catalog Protease Kits & Related Services

    • Targeted Catalog Assay Kits:
      Our portfolio of iD SensoLyteŽ catalog Protease Assay kits feature proteases that have important roles in

      - Neuroscience (Alzheimer's, Parkinson's MS),
      - Cancer,
      - Epigenetics,
      - Cell adhesion & ECM (MMPs),
      - Cardiovascular/Metabolic and
      - Viral pathogenesis.


      Key roles of proteases in structure-function modulation are schematically represented here.
    • Bulk FRET or fluorogenic substrates:
      For developing your own HTS assays, we conveniently offer substrates separately from our assay kits.
    • iD Optimization and Screening:
      includes optimizing and scale up of any SensoLyte® assay kit, and small molecules screening with choice of assay kit.


    Custom Fluorescent Assays & Related Services

    • Design and Development:
      By leveraging our expertise in FRET & Fluorogenic peptide substrate synthesis, and assay development, AnaSpec/EGT group can minimize the assay development time, while delivering the highest quality assay(s), or labeled substrate(s).


    Figure 1. Schematic representation of a protease-based FRET phenomenon leading to the donor's fuorescence recovery upon protease cleavage.

    • Protein/peptide substrate services include:
      Design and synthesis of FRET, TR-FRET and fluorogenic substrates & assays.
    • Validation and screening services include:
      - Validation of assay with inhibitor(s) or biologicals.
      - Screening of small molecules.

    Good to Know!
    Fluorogenic Assays make use of substrates that are labeled with a fluorophore but no quencher. The C-terminal end-specific dyes that are used in this type of assay are quenched by the peptide itself. Upon peptide cleavage by a specific protease, the fluorescence increases.

    TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) uses long-lived fluorescence Lanthanide metal as the donor molecule, such as Europium (Eu), characterized by a large Stoke's shift providing a high signal-to-noise ratio due to minimal crosstalk between excitation and emission wavelengths.

    SensoLyteŽ Catalog Protease Assay Kits

    Protease Category Type Product
    Catalog #
    Neuroscience
    Secretase
    SensoLyteŽ 520 β - Secretase Assay Kit *Fluorimetric*
    SensoLyteŽ 520 TACE (α - Secretase) Activity Assay Kit
    SensoLyteŽ 520 BACE2 Activity Assay *Fluorimetric*
    AS-71144
    AS-72085
    AS-72225
    Neprilysin
    SensoLyteŽ 520 Neprilysin Activity Assay Kit *Fluorimetric*M
    AS-72223
    Calpains
    SensoLyteŽ AMC Calpain Activity Assay Kit *Fluorimetric*
    SensoLyteŽ 520 Calpain Activity Assay Kit *Fluorimetric*
    AS-72150
    AS-72149
    Cathepsins
    SensoLyteŽ Cathepsin Assay kits
    AS-72164;72165
    IDE
    SensoLyteŽ 520 IDE Activity Assay Kit *Fluorimetric*
    AS-72231
    Meprins
    SensoLyteŽ 520 Meprin β Activity Assay Kit *Fluorimetric*
    AS-72254
    Cell Adhesion & ECM
    ADAMTS
    SensoLyteŽ 520 Aggrecanase-1 Assay Kit *Fluorimetric*
    AS-72114
    MMPs
    SensoLyte® MMP Assay Kits
    Cathepsins
    SensoLyte® Cathepsin Assay Kits
    AS-72185:AS-72186
    Elastase
    SensoLyteŽ Rh110 Elastase Assay Kit *Fluorimetric*
    SensoLyteŽ Green Elastase Assay Kit *Fluorimetric*
    AS-72179
    AS-72178
    Cancer & Apoptosis
    Meprins
    SensoLyteŽ 520 Meprin α Activity Assay Kit *Fluorimetric*
    AS-72253
    Caspases
    SensoLyte® Caspases Assay Kits
    AS-71116; 71117; 71120;71121;
    71118;71141;71114;
    Cathepsins
    SensoLyte® Cathepsin Assay Kits
    AS-72170:72098;72222;72171;
    72152;72217:72218;72099:72100
    Matriptase
    SensoLyteŽ Rh110 Matriptase Activity Assay Kit *Fluorimetric*
    AS-72241
    Cardiovascular &
    Metabolic related
    ADAMTS
    SensoLyteŽ 520 ADAMTS13 Activity Assay, *Fluorimetric*
    SensoLyteŽ 520 ADAM10 Activity Assay Kit *Fluorimetric*
    AS-72232
    AS-72226
    Factor Xa
    SensoLyteŽ Rh110 Factor Xa Assay Kit *Fluorimetric*
    SensoLyteŽ 520 Factor Xa Assay Kit *Fluorimetric*
    AS-72207
    AS-72208
    Plasmin & plasminogen
    SensoLyteŽ AFC Plasmin Activity Assay Kit *Fluorimetric*
    SensoLyteŽ Rh110 Plasmin Activity Assay Kit *Fluorimetric*
    AS-72124
    AS-72125
    Renin
    SensoLyteŽ 520 Renin Assay Kit *Fluorimetric*
    SensoLyteŽ 390 Renin Assay Kit *Fluorimetric*
    SensoLyteŽ 520 Rat Renin Assay Kit *Fluorimetric*
    SensoLyteŽ 520 Mouse Renin Assay Kit *Fluorimetric*
    AS-72040
    AS-72039
    AS-72140
    AS-72161
    Thrombin
    SensoLyteŽ 520 Thrombin Activity Assay Kit *Fluorimetric*
    SensoLyteŽ AFC Thrombin Activity Assay Kit *Fluorimetric*
    AS-72129
    AS-72130
    DPPIV
    SensoLyteŽ Rh110 DPPIV Assay Kit *Fluorimetric*
    SensoLyteŽ AMC DPPIV Assay Kit *Fluorimetric*
    AS-72196
    AS-72197
    Enterokinase
    SensoLyteŽ 520 Enterokinase Activity Assay Kit *Fluorimetric*
    SensoLyteŽ Rh110 Enterokinase Activity Assay Kit *Fluorimetric*
    AS-72209
    AS-72210
    Others
    SensoLyteŽ 390 ACE2 Activity Assay Kit *Fluorimetric*
    AS-72086
    Epigenetics
    Enterokinase
    SensoLyteŽ 520 Enterokinase Activity Assay Kit *Fluorimetric*
    SensoLyteŽ Rh110 Enterokinase Activity Assay Kit *Fluorimetric*
    AS-72209
    AS-72210
    Viral & Bacterial Proteases
    HCV Protease
    SensoLyteŽ 490 HCV Protease Assay Kit *Fluorimetric*
    SensoLyteŽ 490 HCV Protease Assay Kit *Fluorimetric*
    SensoLyteŽ 520 HCV Protease Assay Kit *Fluorimetric*
    AS-72087
    AS-71126
    AS-71145
    WNV Protease
    SensoLyteŽ 440 West Nile Virus Protease Assay Kit *Fluorimetric*
    SensoLyteŽ 570 West Nile Virus Protease Assay Kit *Fluorimetric*
    AS-72079
    AS-72080
    Sortase
    SensoLyteŽ 520 Sortase A Activity Assay Kit *Fluorimetric*
    AS-72228
    TEV
    SensoLyteŽ 520 TEV Activity Assay Kit *Fluorimetric*
    AS-72227

    Role of Proteases

    Proteases are enzymes that catalyze the hydrolysis of peptide and isopeptide bonds that link amino acids within proteins, in the process referred to as proteolysis. Proteases have evolved as multifunctional enzymes adapting and diversifying to various biological roles resultant of changes in their expression patterns, substrate specificities and structure-function relationships.Their roles include,


    • Activating cytokines and receptors for cell signaling.

    • Cell death mediators and Antigen processors:
      - Activation of Bid in apoptosis by Caspases-8, Calpains & Cathepsins B, L S, H, D, acting via the loops or interdomain linker regions.
      - Processing antigens via the lysosomal system for presentation by MHC class II molecules, such as cathepsins and deubiquitinating enzymes.

    • Ecdomomain processors by exposing cryptic sites, such as beta-secretase during amyloid precursor protein processing or Shedding various transmembrane proteins such as metalloproteases and cysteine proteases.

    • ECM modulators by using exosites as effective substrate binding,such as thrombin, ADAMTS-4 and most MMPs.

    • Targets for viral inhibitors to arrest viral proteases processing viral polyproteins in causing pathogenicity.
    Figure 2.Schematic representation of diverse roles of Proteases.

    Related Products

    Ordering and related information

    For CUSTOM SERVICES, contact biology@anaspec.com

    For CATALOG PRODUCTS, you may order online or send order request to orders@anaspec.com