SensoLyteŽ Rh110 Plasma Kallikrein Activity Assay kit *Fluorimetric*
AnaSpec_SensoLyteŽ Rh110 Plasma Kallikrein Activity Assay Kit
SensoLyteŽ Rh110 Plasma Kallikrein
ActivityAssay Kit*Fluorimetric*

AS-72255
Kallikrein Assay Kit
SensoLyteŽ Rh110 Plasma Kallikrein Activity Assay Kit is designed to detect human plasma kallikrein in biological fluids.

It also includes a plasma kallikrein inhibitor and protocol which are suited for potential pharmacotherapy studies and compound screening.
Benefits
  • Fast & Convenient
  • Sensitivity 1ng/mL
  • Fluorimetric detection
  • Features
  • detection of plasma kallikrein
  • compound screening for plasma kallikrein inhibitors

  • Plasma Kallikerin detection

    As an important vascular protease/biomarker, Plasma kallikrein represents the kallikrein-kinin system responsible for liberating bradykinin, a potent vasoactive pro-inflammatory peptide. It's detection in plasma lends an important indication of the cardiovascular state involving alternate and classical coagulation pathways.

    Fig 1. Kallikrein activity present in plasma samples was detected by SensoLyteŽ Rh110 Plasma Kallikrein Activity Assay Kit. Plasma samples were diluted by 1X Assay buffer. The fluorescence signal was measured after 1 hour incubation of samples with substrate.
    Fig 2. The specificity of SensoLyteŽ Rh110 Plasma Kallikrein Acitivty Assay Kit was tested with prekallikrein depleted human plasma. Lack of prekallikrein in the depleted plasma resulted in no conversion of prekallikrein to kallikrein.

    Plasma Kallikrein Inhibitors Screening

    Imbalance between plasma kallikrein and its naturally occurring plasma inhibitors is associated with disease states including hereditary angioedema (HAE), systemic lupus, inflammatory bowel disease and rheumatoid arthritis.
    Our kit also provides an effective and convenient way to screen for plasma kallikrein inhibitors in your compound(s) of interest.

    Fig 3. Inhibition of Kallikrein activity by PKSI-527 measured with SensoLyteŽ Rh110 Plasma Kallikrein Activity Assay Kit. Fluorescence signal was detected at Ex/Em=490/520nm (SpectraMax M5e).

    About Kallikrein:

    Plasma Kallikrein is implicated in several processes including blood coagulation, cardiovascular and cerebrovascular physiological and pathological phenomena. It is actively involved in the initiation of classical and activation of the alternate complement pathways.

    Plasma kallikrein is an important vascular protease that represents the kallikrein-kinin proteolytic system responsible for liberating bradykinin, a vasoactive pro-inflammatory peptide.

    Imbalance between plasma kallikrein and its naturally occurring plasma inhibitors is associated with disease states including hereditary angioedema (HAE), systemic lupus, inflammatory bowel disease and rheumatoid arthritis.

    Plasma Kallikrein is considered as a vascular biomarker:
    1. Activated plasma kallikrein initiates surface mediated activation of coagulation, fibrinolysis and kinin generation.
    2. Plasma kallikrein converts prorenin to renin, thus modulating activity of renin angiotensin aldosterone system (RAAS) at various levels.
    3. Plasma kallikrein accelerates activation of FXII towards fibrinolytic mechanism.


    References
    1. Costa-Neto CM, Dillenburg-Pilla P, Heinrich TA, Parreiras-e-Silva LT, Pereira MG, Reis RI, Souza PP. Participation of kallikrein-kinin system in different pathologies. Int Immunopharmacol. 2008;8(2):135-42.

    2. Bryant JW, Shariat-Madar z. Human plasma kallikrein-kinin system: Physiological and biochemical parameters. Cardiovascular & hematological agents in medicinal chemistry. 2009;7(3):234-250.

    3. Kolte D, Shariat-Madar Z. Plasma Kallikrein Inhibitors in Cardiovascular Disease: An Innovative Therapeutic Approach. Cardiol Rev. 2016;24(3):99-109.