and streptavidin show extraordinary strong affinity to biotin. The dissociation
constant (Kd) of the non-covalent interaction between streptavidin and biotin
is on the order of 10-15 M. The streptavidin-biotin complex is
also highly stable over a wide range of temperatures and pH. As a result,
the streptavidin-biotin interaction has been extensively used in molecular
biology, immunology, and cellular assays.
is pleased to introduce our latest collection of streptavidins labeled with
our patented HiLyte™ Fluor and HiLytePlus™ dyes. They
offer brighter fluorescence and better photostability compared to FITC and
dyes. Streptavidins labeled with fluorescent dyes are widely used for immunofluorescent
staining (Fig.1, 2), fluorescence activated cell sorting, in situ hybridization
and other biological applications.
Fluor Labeled Streptavidin Sampler kits, available in 2 sizes,
contain streptavidins labeled individually with HiLyte™ Fluor.
Prepared at optimal fluorophore to streptavidin labeling ratio,
these HiLyte™ Fluor streptavidin conjugates exhibit high fluorescence signal
and uncompromised biotin binding capability. Every lot has been validated
with immunofluorescence staining to guarantee the best performance.
conjugated to classic dyes such as FAM, TAMRA, as well as B-PE, R-PE and APC
are also available.
here to request additional information
HRP conjugated Streptavidins:
N - (Biotinoyl) -
N'' - (iodoacetyl)ethylenediamine
Figure 1. Alpha-tubulin in bovine pulmonary artery endothelial
cells were detected with biotin-conjugated mouse anti alpha-tubulin primary
antibody, visualized with HiLyte™ Fluor 647 conjugated streptavidin (Cat#60667).
Mitochondria were stained with mouse anti Oxphos complex V primary antibody,
visualized with HiLyte™ Fluor 488-conjugated goat anti mouse IgG (Cat# 28175-H488).
Nucleus was stained with DAPI (Cat# 83210).
Actins in bovine pulmonary artery endothelial cells were detected with biotin-conjugated
phalloidin, visualized with R-phycoerythrin-conjugated streptavidin (Cat#60669).
The nuclei were stained with Hoechst 33342 (Cat# 83218).