PKD-1 & PKD-2 Antibodies Isoform Specific


Protein kinase D (PKD), a serine/threonine kinase originally described as a novel PKC family member and termed PKCm (1), belongs to the calcium calmodulin superfamily of kinases (2-3). Three mammalian isoforms have so far been described - PKD1/PKCm, PKD2 and PKD3/PKCn; these isoforms show a high degree of homology, especially in their catalytic domain (4-5). PKDs are major targets for tumor promoting phorbol esters; they are activated via G protein-coupled receptors (GPCRs) and their activation is also dependent on PKC activation (5). PKDs have been implicated in numerous cellular functions, including signal transduction as well as cell survival, migration, differentiation, and proliferation (4-6). They are important regulators of secretory transport at the trans-golgi network (7). Of the three isoforms, PKD1 is the best characterized. It is involved in the regulation of Golgi function, cell proliferation and apoptosis (8) and it mediates oxidative stress signaling regulating cellular detoxification and survival (9). PKD2 has been found to phosphorylate histone H1 more efficiently than aldolase in vitro (10).

AnaSpec is pleased to announce the release of isoform specific PKD1 and PKD2 antibodies. These antibodies were raised using sequences from the C-terminus of PKD1 and PKD2. Anti-PKD1 only recognizes PKD1 and not PKD2 and PKD3. Anti-PKD2 only recognizes PKD2 and not PKD1 or PKD3.

Anti - PKD1 kinase (CT), humanNEW

50 g

55141

Anti - PKD2 Kinase (CT), humanNEW

50 g

55142

Figure 1: Western blots: HEK293T cells were transfected with HA-tagged human PKD1, Flag-tagged human PKD2 and GST-tagged human PKD3 and incubated at 37C for 24 hours. The cultures were then lysed and run on a 7.5% SDS-PAGE gel. Blots probed with anti-HA, -Flag (anti-DYKDDDDK Tag) and anti-GST at a final concentration of 200 ng/ml in 5% milk in TBST at 4C overnight. Courtesy of Dr. Alex Toker, Harvard Medical School, Boston, MA.

Figure 2. Western blots: HEK293T cells were transfected with HA-tagged human PKD1, Flag-tagged human PKD2 and GST-tagged human PKD3 and incubated at 37C for 24 hours. The cultures were then lysed and 100 ug of total lysate run on a 7.5% SDS-PAGE gel. Blots were then probed with -human PKD2 kinase (Cat# 55142), and control -HA, anti-Flag (anti-DYKDDDDK Tag) and -GST at a final concentration of 200 ng/ml in 5% milk in TBST at 4C overnight. Courtesy of Dr. Alex Toker, Harvard Medical School, Boston, MA.

Related Products:

Other Protein Kinase antibodies.
Large selection of kinase/phosphatase peptide substrates

References

1.Johannes, FJ. et al. J. Biol. Chem. 269, 6140 (1994).
2.Manning, G. et al. Science 298, 1912 (2002).
3.Van Lint, J. et al. Trends Cell Biol. 12, 193 (2002).
4.Waldron, RT. et al. J. Biol. Chem. 276, 32606 (2001).
5.Auer, A. et al. Mol. Bio. Cell. 16, 4375 (2005).
6.Rozengurt, E. et al. J. Biol. Chem. 208, 13205 (2005).
7.Yeaman, C. et al. Nature Cell Biol. 6, 106 (2004).
8.Rykx, A. et al. FEBS Lett. 546, 81 (2003).
9.Dppler, H. & P. Storz, et al. J. Biol. Chem. 282, 31873 (2007).
10.Sturany, S. et al. J. Biol. Chem. 276, 3310 (2001).