a leader in phosphopeptide synthesis, is proud to introduce two phosphospecific
IRAK1 polyclonal antibodies – anti-IRAK1 (pThr 209)
(pThr387). As seen on the dot blots (Figures 1-2), these antibodies are
specific for the phosphothreonine sequence and not the non-phosphothreonine
sequence. As well, the cross-reactivity between the pThr209 and pThr 387 antibodies
is minimal (Figure 3).
(IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase
(1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2,
IRAK3/IRAK-M and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI)
initiates the formation of a complex that includes IL-1RI, AcP, MyD88 and IRAKs
(2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation and
serves as a signaling molecule to mediate IL-1 response (3). IRAK mediates a
signaling cascade leading to NF-KB activation by members in IL-1 family including
IL-1 and a novel cytokine IL-18 (also termed IGIF) (1, 4).
– cat# 55331 polyclonal antibody was raised against a peptide corresponding
to human IRAK1 at the phosphorylated threonine 209 (EISRGpTH), while Rabbit anti-IRAK-1(pThr387)
– cat# 55333 polyclonal antibody corresponds to human IRAK1 at the phosphorylated
threonine 387 (VRG-pTLAY). The non-phosphorylated
version of these antibodies is also available. The IRAK1 antibodies have
been evaluated for specificity by dot blot.
Click here to
request more information.
||Figure 1. Dot blot of anti-IRAK1 (pThr209) and
anti-IRAK1 (paired pThr209 – non-phosphorylated).
||Figure 2. Dot blot of anti-IRAK1 (pThr387) and anti-IRAK1
(paired pThr387 – non-phosphorylated).
||Figure 3. Dot blot of anti-IRAK1 (pThr209) and anti-IRAK1
(paired pThr209 – non-phosphorylated) and anti-IRAK1 (pThr387) and
anti-IRAK1 (paired pThr387 – non-phosphorylated).
Kinase & Phosphatase Peptide Substrates
1. Dinarello, CA. Blood
87, 2095-2147 (1996).
2. Takaesu, G. et al. Mol. Cell.
Biol. 21, 2475-2484 (2001).
3. Jianing H. et al. Proc. Natl.
Acad. Sci. USA 94, 12829 (1997).
4. Robinson D. et al. Immunity
7, 571 (1997).
pleased to introduce new IRAK1 phosphothreonine 209 and 387 antibodies.