New Z-Fish™ Antibodies


Zebrafish (Danio rerio) as an experimental model has gained widespread use since the pioneering days of Dr. George Streisinger. In the year 1980, there were 18 publications, 72 in 1990, 540 in 2000 and 1159 in 2008.1 To date, there are more than 13,200 publications.2 In order to address the growing needs of the research community for zebrafish specific antibodies, peptides and other products, AnaSpec is pleased to announce the launching of the Z-Fish™ product line.

Z-Fish™ antibodies are raised strictly from peptide antigens derived from zebrafish protein sequences, i.e. 100% zebrafish sequence homology. Leveraging AnaSpec’s expertise in peptide technology, the antigens used in raising these antibodies include non-phosphorylated and phosphorylated peptides. The antibodies are purified using proprietary purification methods.

The list of Z-Fish™ antibodies includes targets used in apoptosis and autophagy, cell cycle control, cytoskeleton, glucose and energy metabolism, signal transduction, and immunity research. Antibodies were verified in ELISA and in western blots, using zebrafish lysates. Z-Fish™ antibodies are supplied in 150 ul sizes and are enough for 15 mini gels.

Z-Fish™ cytoskeleton antibodies include anti-actin, α-smooth muscle, anti-beta-actin, raised with peptide antigens either from the C or N-terminus of the protein, anti-keratin, and β-Catenin, an actin related product. β-Catenin, a protein that bridges between cadherins and actin and plays a central role in cell adhesion,can be phosphorylated by GSK-3.3-6 One phosphorylated and two non-phosphorylated antibodies are available for this protein.

Anti-Actin, Z-Fish

Size

Cat #

Anti - Actin, alpha smooth muscle, Z - Fish™ NEW
Host: rabbit polyclonal; Species reactivity: zebrafish; Application: WB, IHC (predicted)

150 ul/15 mini-WB

55340

Anti - Actin - beta (CT), Z - Fish™ NEW
Host: rabbit polyclonal; Species reactivity: zebrafish; Application: WB, IHC (predicted)

150 ul/15 mini-WB

55339

Anti - Actin - beta (NT), Z - Fish™ NEW
Host: rabbit polyclonal; Species reactivity: zebrafish; Application: WB, IHC (predicted)

150 ul/15 mini-WB

55338


Anti-Keratin, Z-Fish™

Size

Cat #

Anti - keratin - 18 (IN - 1), Z - Fish™ NEW
Host: rabbit polyclonal; Species reactivity: zebrafish; Application: WB, IHC (predicted)

150 ul/15 mini-WB

55357


Anti-β-Catenin, Z-Fish™

Size

Cat #

Anti - Catenin - beta - 2 (IN), Z - Fish™ NEW
Host: rabbit polyclonal; Species reactivity: zebrafish; Application: WB, IHC (predicted)

150 ul/15 mini-WB

55365

Anti - Catenin - beta - 2 (NT), Z - Fish™ NEW
Host: rabbit polyclonal; Species reactivity: zebrafish; Application: WB, IHC (predicted)

150 ul/15 mini-WB

55364

Anti - Catenin - beta 1, 2 (paired674), Z - Fish™ NEW
Host: rabbit polyclonal; Species reactivity: zebrafish; Application: WB, IHC (predicted)

150 ul/15 mini-WB

55366







For custom production of zebrafish antibodies, phosphorylated or non-phosphorylated, please contact AnaSpec (service@anaspec.com).

Related Products:

Full listing of Z-Fish™ antibodies
Z - Fish™ Embryonic Lysate (24 hpf) NEW
Z - Fish™ Embryonic Lysate (48 hpf) NEW
Z - Fish™ Embryonic Lysate (72 hpf) NEW
Z - Fish™ Lysate (5 dpf) NEW
Full listing of antibodies

References

  1. ZFIN; Retrieved Apr. 15, 2009 from http://nar.oxfordjournals.org/cgi/reprint/31/1/241.
  2. Highwire; Retrieved Apr. 15, 2009 from http://highwire.org.
  3. Dale, TC. Biochem. J. 329, 209 (1998).
  4. Sadot, E. et al. J. Cell Sci. 115, 2771 (2002).
  5. Giannini, A. et al. J. Biol. Chem. 275, 21883 (2000).
  6. Thilo, H. et al. J. Biol. Chem. 277, 2330 (2002).