Anti-p53 Antibodies - Z-Fish™ and Human


AnaSpec is pleased to announce the release of a zebrafish specific p53 antibody, the Z-Fish™ Anti-p53. This antibody was tested in fluorescent western blot analysis using HiLyte™ Fluor 750 shows an immunoreactive band 53 kD, this band was also abrogated upon addition of the immunizing peptide (Figure 1).

Product

Size

Catalog #

Anti-p53 (CT), Z-Fish™ NEW
Host: rabbit polyclonal; Species reactivity: zebrafish; Application: WB

150 ul/15 mini-WB

55342



Figure 1. Fluorescent western blot analysis of zebrafish lysate (5 dpf) with Z-Fish™ Anti-p53 (CT), cat# 55342. An imunoreactive band at ~53 kDa was detected (A); this band was blocked by the immunizing peptide (B). HiLyte™ Fluor 750 –conjugated secondary antibody was used and the signals were detected with an infrared-imaging system.


Anti-p53, Human or Mouse Specific Antibodies

One of the most important mammalian cell cycle checkpoint proteins is the tumor suppressor protein, p53. In normal, undamaged cells, p53 is rapidly degraded. However, when cells are treated with DNA damage-inducing agents, there is a transient accumulation of p53 protein and it is activated as a transcription factor. In several types of human cancers, p53 is mutated.1,2 Human p53 protein has been shown to be phosphorylated at several N-terminal and C-terminal sites that affect site-specific DNA binding and interaction with other cellular and viral proteins in vitro.3-8 Phosphorylation at serines 6, 9, 15, 20, 33, 37 occurs after cells are exposed either to ionizing radiation or to UV light.9,10 Serines 6 and 15 were demonstrated to be among the strongest and earliest phosphorylated sites in response to DNA damage-induced posttranslational modifications.11,12 AnaSpec’s collection of p53 antibody products includes both phosphospecific and non-phosphospecific solutions.

Product

Size

Catalog #

Anti-p53 (CT)
Host:rabbit polyclonal; Species Reactivity: human, mouse, rat; Application: WB

50 µg

54421

Anti-p53 (pSer6)
Host: rabbit polyclonal; Species Reactivity: human; Application: WB

25 µg

50 µg

28006-025

28006

Anti-p53 (paired6)
Host: rabbit polyclonal; Species Reactivity: human; Application: WB

50 µg

28007

Anti-p53 (pSer9)
Host: rabbit polyclonal; Species Reactivity: human; Application: WB

25 µg

50 µg

28008-025

28008

Anti-p53 (paired9)
Host: rabbit polyclonal; Species Reactivity: human; Application: WB

50 µg

28009

Anti-p53 (pSer15)
Host: rabbit polyclonal; Species Reactivity: human; Application: WB

25 µg

50 µg

28010-025

28010

Anti-p53 (paired15)
Host: rabbit polyclonal; Species Reactivity: human; Application: WB

50 µg

28011

Anti-p53 (pSer20)
Host:rabbit polyclonal; Species Reactivity: human; Application: WB

25 µg

50 µg

54427-025

54427

Anti-p53 (paired20)
Host:rabbit polyclonal; Species Reactivity: human, monkey; Application: WB

50 µg

55014

Anti-p53 (pSer37)
Host:rabbit polyclonal; Species Reactivity: human; Application: Dot blot

25 µg

50 µg

54428-025

54428

Anti-p53 (paired37)
Host:rabbit polyclonal; Species Reactivity: human, monkey; Application: WB

50 µg

55015

Anti-p53 (pSer46)
Host:rabbit polyclonal; Species Reactivity: human; Application: Dot blot

25 µg

50 µg

54430-025

54430

Anti-p53 (paired46)
Host:rabbit polyclonal; Species Reactivity: human, monkey; Application: WB

50 µg

55016

Anti-p53 (pSer392)
Host:rabbit polyclonal; Species Reactivity: human; Application:Dot blot

25 µg

50 µg

54429-025

54429

Anti-p53 (paired392)
Host:rabbit polyclonal; Species Reactivity: human, monkey; Application: WB

50 µg

55017

Anti-p53 (pSer18/23), mouse
Host: rabbit polyclonal; Species Reactivity: mouse; Application: Dot blot

50 µg

55038

Anti-p53 (paired18/23), mouse
Host: rabbit polyclonal; Species Reactivity: mouse, monkey; Application: WB

50 µg

55039

Anti-p53 (pSer23), mouse
Host: rabbit polyclonal; Species Reactivity: mouse; Application: Dot blot

50 µg

55036

Anti-p53 (paired23), mouse
Host: rabbit polyclonal; Species Reactivity: mouse, monkey; Application: WB

50 µg

55037

Anti-p53 (pSer18), mouse
Host: rabbit polyclonal; Species Reactivity: mouse; Application: WB

50 µg

55034A

Anti-p53 (paired18), mouse
Host: rabbit polyclonal; Species Reactivity: mouse, monkey; Application: WB

50 µg

55035A


References:

1. Brown, JM. et al. Cancer Res. 59, 1391(1999).
2. Albrechtsen, N. et al. Oncogene 18, 7706 (1999).
3. Wang, L. et al. J. Biol Chem. 276, 43604 (2001).
4. Xirodimas, D. et al. Oncogene 20, 4972 (2001).
5. Backlund, MG. et al. Cancer Res. 61, 6577 (2001).
6. Sakaguchi, K. et al. J. Biol. Chem. 275, 9278 (2000).
7. Banin, S. et al. Science 281, 1674 (1998).
8. Canman, CE. et al. Science 281, 1677 (1998).
9. Burns, TF. and El-Deiry, WS. J. cell Physiol. 181, 231 (1999).
10. Oren, M. et al. J. Biol. Chem. 274, 36031 (1999).
11. Lakin, ND. et al Oncogene 18, 7644 (1999).
12. Higashimoto, Y. et al. J. Biol. Chem. 275, 23199 (2000).