SensoLyte® 520 MMP Assay Kits – ultra-sensitive


AnaSpec’s SensoLyte® MMP assay kits, especially the SensoLyte® 520 MMP Assay Kits, have become the new standard in MMP assay sensitivity. With published results in world-class journals and the industry’s longest wavelength, the SensoLyte® 520 line is one of the most sensitive (Figure 1) and brightest lines of MMP assay kits in the world.

SensoLyte® 520 MMP Assays

• Measure MMP activity
• 96-well or 384-well format
• Mix and read
• Fluorimetric - long wavelength
• 5-FAM/QXL™ 520 FRET pair (Ex/Em=490/520 nm)
• Minimal autofluorescence
• Assay time – as short as 1h

Figure 1. The performance of an identical peptide sequence, one coupled with 5-FAM/QXLTM 520 and the other coupled with EDANS/DABCYL FRET pairs was compared (A). Fluorescence was measured after 1 hr incubation of FRET substrates with serial dilutions of MMP-13 (Flex800, Bio-Tek instruments). A 5-FAM/QXLTM 520 MMP substrate was compared with Mca/Dpa FRET substrate (B). Fluorescence was measured after 1 hr incubation of substrates using serial dilutions of MMP-13 (Flexstation 384II, Molecular Devices).

Table 1. A listing of the MMP assay kits and recombinant MMPs

SensoLyte® 520 Series

SensoLyte Plus® 520 Series

SensoLyte® 490 Series

SensoLyte® ELISA Series

Recombinant Human MMPs

MMP-1

71150

72012

71128

72102

72004

MMP-2

71151

71129

72005

MMP-3

71152

71130

72103

72006

MMP-7

71153

71132

72007

MMP-8

71154

71133

72104

72008

MMP-9

71155

72017

71134

72105

72009

72069 (mouse)

MMP-10

72024

72106

72067

MMP-12

71157

71137

72010

MMP-13

71156

72019

71135

72107

72011

MMP-14

72025

72068

MMP-15

72198

MMP-16

72200

MMP-24

72201

SensoLyte® Plus 520 MMP Assays

• Measure MMP activity
• Enhanced specificity – human capture antibody included
• 96-well format (strips)
• Mix and read
• Fluorimetric - long wavelength
• 5-FAM/QXL™ 520 FRET pair (Ex/Em=490/520 nm)
• Minimal autofluorescence