AnaSpec,
EGT Group is pleased to announce the release of the industry first fluorimetric
Factor Xa activity assays - The
SensoLyte® 520 Factor Xa Assay Kit
and the
SensoLyte® Rh110 Factor Xa Assay Kit. The
SensoLyte® 520 Factor Xa Assay Kit
employs a FRET (5-FAM/QXL™ 520) substrate and the
SensoLyte® Rh110 Factor Xa Assay Kit,
a substrate labeled with the fluorophore, Rh110. Both substrates are
fluorescent substrates emitting in the green range of 520 nm, with excitation
at 490 nm. The longer-wavelength spectra of 5-FAM and Rhodamine 110 provide
greater sensitivity and less interference from other reaction components, making
these kits ultra-sensitive. The detection limit of the SensoLyte® 520 kit can
reach as low as 7.8 ng/mL while the SensoLyte® Rho110 reaches as low as 0.31
ng/mL.
• Kits optimized to detect Factor Xa enzyme activity
• Fluorometric, long wavelength (Ex/Em=490/520 nm)
read-out assays
• Minimal
autofluorescence
• Kits provide ample
reagents to perform 100 assays in a 96-well plate format
• Assays can be completed
in one hour
Factor
Xa (FXa) is a serine endopeptidase composed of two disulfide-linked subunits.
FXa leads to blood clot formation by converting prothrombin to thrombin through the prothrombinase complex.
FXa is generated from zymogen Factor X via the intrinsic and extrinsic pathways
and is the rate-limiting step in the propagation of thrombin generation. In
the presence of Ca2+ ions, FXa forms prothrombinase with factor Va
on the phospholipid membrane of the activated platelets.1-3 FXa has
emerged as an attractive target for drug discovery for thromboembolic diseases.4,5
Figure 1. Inhibition of Factor Xa activity as measured with SensoLyte®
Rh110 Factor Xa Assay Kit.

Figure 2. Inhibition of Factor Xa activity as measured with the SensoLyte®
520 Factor Xa Assay Kit.
Related Products
Reference
1. Furie, B. and Furie, BC. Cell 53:505–518
(1988).
2. Davie, EW. et al. Biochemistry 30:10363–10370(1991).
3. Mann, KG. Thromb Haemost 82:165-174 (1999).
4. Camm, AJ. and Bounameaux, H. Drugs 71:1503-1526
(2011).
5. Weitz, JI. Thromb Res 127 Suppl 2:S5-S12 (2011).
|