Anti-His & Anti-GPF Antibodies

The introduction of tags, whether short peptides (6X-His, DYKDDDDK, c-myc) or proteins (green fluorescent protein – GFP, glutathione-s-transferase – GST) into recombinant proteins aids in the characterization, localization or purification of the recombinant expressed protein.1-3 As a consequence, antibodies raised against fusion tags are useful in biochemical or immunohistochemistry applications.

AnaSpec, EGT Group is pleased to offer a wide selection of high quality monoclonal and polyclonal antibodies against the hexa-His and GFP tags.

Wide selection of anti-His and anti-GFP antibodies
Polyclonal and monoclonal antibodies
Fluorophore or biotin-labeled His tag antibodies available

Table 1. Anti-His tag antibodies are available as polyclonal or monoclonal antibodies

Product

Size

Catalog #

Anti-His-tag (polyclonal)

100 µg

500 µg

1 mg

AS-29673

AS-29673-500

AS-29673-1000

Anti-His-tag (monoclonal)

50 µg

AS-61250


Table 2. A listing of Anti-GFP antibodies.

Product

Size

Catalog #

Anti-GFP (1A5), monoclonal

100 ug

AS-55887

Anti-GFP Tag (6A2B3), monoclonal

100 ug

AS-55886

Anti-GFP Tag

100 ug

500 ug

1 mg

AS-29779

AS-29779-500

AS-29779-1000

Anti-GFP Tag (CT )

100 ug

AS-53882

Anti-GFP Tag (NT)

100 ug

AS-54045

Anti-GFP Tag (IN)

100 ug

AS-53214

Anti-GFP-Tag, Chicken IgY

100 ug

AS-55423



Figure 1. ICC of HEK293 cells transfected with GFP and probed with polyclonal Anti-GFP (Cat# 29779). Secondary antibody used was Goat anti-rabbit, Hilyte™ Fluor 555 labeled (red). Nucleus counter-stained with DAPI.

Fluorescently labeled and biotin labeled 6X-His antibodies are also available. Antibodies are labeled with a selection of dyes, including our proprietary HiLyte™ Fluor dyes, which span the visible spectrum. This gives researchers a wide range of wavelength choices for use in immunohistochemistry or immunofluorescence applications.

Table 3. A listing of biotin and dye-labeled polyclonal Anti-His antibodies.

Product

Size

Catalog #

Polyclonal Anti-His Tag IgG, Biotinylated

100 µg

AS-29673-BIOT

Polyclonal Anti-His Tag IgG, FITC labeled (Ex/Em=495/520 nm)

100 µg

AS-29673-FITC

Polyclonal Anti-His Tag IgG, HiLyte™ Fluor 405-labeled (Ex/Em=404/428 nm)

100 µg

AS-29673-H405

Polyclonal Anti-His Tag IgG, HiLyte™ Fluor 488 labeled (Ex/Em=499/523 nm)

100 µg

AS-29673-H488

Polyclonal Anti-His Tag IgG, HiLyte™ Fluor 555 labeled (Ex/Em=553/568 nm)

100 µg

AS-29673-H555

Polyclonal Anti-His Tag IgG, HiLyte™ Fluor 594 labeled (Ex/Em=593/616 nm)

100 µg

AS-29673-H594

Polyclonal Anti-His Tag IgG, HiLyte™ Fluor 647 labeled (Ex/Em=653/673 nm)

100 µg

AS-29673-H647



Table 4. A listing of biotin and dye-labeled monoclonal Anti-His antibodies.

Product

Size

Catalog #

Monoclonal Anti-His Tag IgG, Biotinylated

100 µg

AS-61250-BIOT

Monoclonal Anti-His Tag IgG, AMCA labeled (Ex/Em=344/446 nm)

100 µg

AS-61250-AMCA

Monoclonal Anti-His Tag IgG, FITC labeled (Ex/Em=495/520nm)

100 µg

AS-61250-FITC

Monoclonal Anti-His Tag IgG, TAMRA labeled (Ex/Em=555/580 nm)

100 µg

AS-61250-TAMR

Monoclonal Anti-His Tag IgG, HiLyte™ Fluor 488 labeled (Ex/Em=499/523 nm)

100 µg

AS-61250-H488

Monoclonal Anti-His Tag IgG, HiLyte™ Fluor 555 labeled (Ex/Em=553/568 nm)

100 µg

AS-61250-H555

Monoclonal Anti-His Tag IgG, HiLyte™ Fluor 647 labeled (Ex/Em=653/673 nm)

100 µg

AS-61250-H647


Related Products
Full listing of tag antibodies

References:
1. Zheng et al. J Biochem 133, 577 (2003).
2. Amrein et al. PNAS 92, 1048 (1995).
3. Nakajima K. and Yaoita Y. Nucleic Acids Res 25, 2231 (1997).
4. Medina et al. Nucleic Acids Res 28, I (2000).