Lysosomal Sorting of Amyloid-β by the SORLA Receptor Is Impaired by a Familial Alzheimer’s Disease Mutation includes the most recent citation for our HiLyte Fluor™ labeled β-amyloid peptide.
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- Caglayan S, et al. (2014). Lysosomal Sorting of Amyloid-β by the SORLA Receptor Is Impaired by a Familial Alzheimer’s Disease Mutation. Sci. Transl. Med. 6, 223ra20. DOI: 10.1126/scitranslmed.3007747
SORLA/SORL1 is a neuronal sorting receptor for the amyloid precursor protein that has been implicated in both the sporadic and autosomal dominant forms of Alzheimer’s disease (AD). SORLA concentrations are inversely correlated with β-amyloid in mouse models and AD patents. Caglayan characterized a new mouse model in which SORLA is overexpressed and showed a decrease in β-amyloid concentration in mouse brain. β-amyloid concentration was determined using AnaSpec’s HiLyte Fluor™ labeled β-amyloid peptide.
- Zeng Y, et al. (2014). Sphingosine-1-phosphate protects endothelial glycocalyx by inhibiting syndecan-1 shedding. Am J Physiol Heart Circ Physiol. 306, H363-H372. DOI: 10.1152/ajpheart.00687.2013
Sphingosine-1-phosphate (S1P) in albumin is thought to stabilize surface glycocalyx which is responsible for blood-tissue interface functions. The mechanism of protection from loss of glycocalyx components by S1P-dependent pathways is shown to suppress metalloproteinase (MMP) activity. MMP activity was measured using AnaSpec’s SensoLyte® 390 Generic MMP Assay kit. The researchers were able to conclude that S1P plays a critical role in protecting the glycocalyx via S1P receptors, and inhibits the protease activity-dependent shedding of CS, HS, and the syndecan-1 ectodomain.
- Kanda A, et al. (2013). Atp6ap2/(Pro)renin Receptor Interacts with Par3 as a Cell Polarity Determinant Required for Laminar Formation during Retinal Development in Mice. J Neurosci. 49, 19341-19351. DOI: 10.1523/JNEUROSCI.1362-13.2013
(Pro)renin receptor also known as Atp6ap2, is a key molecule in the tissue renin-angiotensin system (RAS) that plays a role in tissue RAS activation and as a subunit for the vacuolar H+-ATPase proton pump. Kanda’s group studies the novel function of Atp6ap2 required for laminar formation during retinal development. Using AnaSpec’s HiLyte Fluor™ 647 fluorescent dye, they were able to perform IHC experiments to visualize their sections. They postulate that this cellular activity associated with the Par-aPKC system, in addition to the v-ATPase function and tissue RAS activation, is the third biological role of Atp6ap2.
- Hoffmann C, et al. (2014). Live cell imaging reveals actin-cytoskeleton-induced self-association of the actin-bundling protein WLIM1. J Cell Sci. 127, 582-598. DOI: 10.1242/jcs.134536
Hoffmann’s team studies the crosslinking of actin filaments into bundles and the molecular mechanisms underlying actin bundle formation. Using our proprietary HiLyte Fluor™ 488 dye in conjunction with FLIM-FRET, the team was able to develop a model to see how actin binding promotes the formation and stabilization of NtWLIM1 complexes, which is hypothesized to drive the crosslinking of actin filaments.
- Quinn SD, et al. (2014). Real-time probing of β-amyloid self-assembly and inhibition using fluorescence self-quenching between neighbouring dyes. Mol. BioSyst. 10, 34-44. DOI: 10.1039/C3MB70272C
Quinn's group characterized the fluorescence response of AnaSpec’s HiLyte Fluor™ 555-labelled Aβ peptides and demonstrated that Aβ self-assembly organizes the covalently attached probes in close proximity to trigger the self-quenching sensing process over a broad range of conditions. Additionally, they proved that N-terminal tagging of β-amyloid peptides does not alter the self-assembly kinetics or the resulting aggregated structures.
- Bush CO, et al. (2014). A Small-Molecule Inhibitor of Hepatitis C Virus Infectivity. Antimicrob. Agents Chemother. 58, 386-396. DOI: 10.1128/AAC.02083-13
Bush C., et al. from Gilead Sciences, Inc. performs TR-FRET experiments using AnaSpec's NS3-4A europium substrate for Time-Resolved Fluorescence. NS3-4A protease activity was used to monitor intracellular HCV replication levels and was measured by using a europium-labeled NS3-4A protease substrate.
- Scruggs BA, et al. (2013). Age of the Donor Reduces the Ability of Human Adipose-Derived Stem Cells to Alleviate Symptoms in the Experimental Autoimmune Encephalomyelitis Mouse Model. Stem Cells Trans Med. 2, 797-807. DOI: 10.5966/sctm.2013-0026
Mice were induced with chronic experimental autoimmune encephalomyelitis (EAE) using the myelin oligodendrocyte glycoprotein35–55 peptide and treated before disease onset with ASCs derived from younger (<35 years) or older (>60 years) donors. AnaSpec’s MOG 35–55 peptide was administered to the mice to induce chronic EAE, which models PPMS more than other MS forms.
- Semon JA, et al. (2013). Administration of Murine Stromal Vascular Fraction Ameliorates Chronic Experimental Autoimmune Encephalomyelitis. Stem Cells Trans Med. 2, 789-796. DOI: 10.5966/sctm.2013-0032
The ability of murine SVF cells to treat myelin oligodendrocyte glycoprotein 35–55-induced experimental autoimmune encephalitis (EAE) was compared with that of culture-expanded ASCs in C57Bl/6J mice. Chronic EAE was induced in these animals by subcutaneous immunization using AnaSpec’s MOG 35–55 peptide.
- Somarowthu S, et al. (2013). Visualizing the ai5γ group IIB intron. Nucl. Acids Res. 1-12. DOI: