Although FITC reagents have been predominantly used to prepare green fluorescent bioconjugates, the low photostability and pH-dependent fluorescence of FITC bioconjugates make researchers to search for alternative fluorophores in bioconjugations. Among green fluorophores, CR110 reagents give more photostable and pH-independent bioconjugates that have the absorption maximum around the preferred 488 nm excitation wavelength. 5 (6)-CR110, SE is an excellent amino-reactive CR110-based labeling reagent.
Xiao M and Kwok PY (2003). DNA analysis by fluorescence quenching detection. Genome Res 13, 932-9; Hung SC, et al. (1998). Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations. Anal Biochem 255, 32-8.