5-FAM-X, SE contains a seven-atom aminohexanoyl spacer (known as ‘X’) between the FAM fluorophore and the succinimidyl ester. The ‘X’ spacer separates the fluorophore from the biomolecule to which it is conjugated. The ‘X’ spacer may potentially reduce the quenching that typically occurs upon conjugation. We recommend this fluorescein derivative as the preferred dye for preparing fluoresceinated proteins when the fluorescence quenching of the labeling dyes by proteins is a serious problem.
Czapski GA, et al. (2001). Detection of protein oxidation in endothelial cells by fluorescently labeled tyramine. Med Sci Monit 7, 606-9; Cooper WC, et al. (2000). Detection of fluorescently labeled actin-bound cross-bridges in actively contracting myofibrils. Biophys J 78, 1449-57.
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