HiLyte Fluor™ 488 hydrazide is a carbonyl-reactive fluorescent labeling dye. It can be used for labeling glycoproteins such as HRP. Fluorescence of HiLyte Fluor™ 488 fluorophore is independent of pH from 4 to 10. This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only above pH 10.
Extinction Coefficient (M-1cm-1): 70,000 Fluorescence quantum yield: 0.91 Fluorescence Life Time (ns): 4.1