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Fluorescent Dyes  >  Protein Phosphorylation and Signal Transduction  >>  Fluorescein diphosphate, tetraammonium salt (FDP)

Product Name Fluorescein diphosphate, tetraammonium salt (FDP)
Size 5 mg
Catalog # AS-85300
US$ $121

Fluorescein diphosphate (FDP) is one of the most sensitive fluorogenic phosphatase substrates commercially available. The colorless and nonfluorescent FDP is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Alternatively, FDP can also be used to detect phosphatases in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). FDP has been widely used in various ELISA assays. Additionally it is also used to detect tyrosine phosphatases. FDP is not very thermally stable, and special cautions need be excised for storing the solid sample and stock solutions.

Detailed Information Material Safety Data Sheets (MSDS)
Storage -20C desiccated and protected from light
References Nolkrantz K, et al. (2002). Functional screening of intracellular proteins in single cells and in patterned cell arrays using electroporation. Anal Chem 74, 4300-5. Murakami Y, et al. (2001). On-chip capillary electrophoresis for alkaline phosphatase testing. Biosens Bioelectron 16, 1009-14. Huang Z, et al. (1992). A sensitive competitive ELISA for 2,4-dinitrophenol using 3,6-fluorescein diphosphate as a fluorogenic substrate. J Immunol Methods 149, 261-6.
Molecular Weight 560.39
Spectral Properties Abs/Em = 272/NA nm
Solvent System Water or DMSO
Product Citations Krueger, AB. et al. (2013). Identification of a selective small-molecule inhibitor series targeting the Eyes Absent 2 (Eya2) phosphatase activity. J Biomol Screen 18, 85. doi: 10.1177/1087057112453936.

Wilson, M. et al. (2012). Picomolar concentrations of free zinc(II) ions regulate receptor protein tyrosine phosphatase beta activity. J Biol Chem 287, 3922. doi: 10.1074/jbc.C111.320796.

Murthy, V. et al. (2009). Enzyme encapsulation using nanoparticle-assembled capsules. Biomol Catalysis doi: 10.1021/bk-2008-0986.ch013.

Herrmann, M. et al. (2007). Microfluidic ELISA on non-passivated PDMS chip using magnetic bead transfer inside dual networks of channels. Lab Chip 7, 1546. doi: 10.1039/b707883h.
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