SensoLyte® FDP Alkaline Phosphatase ELISA Assay Kit *Fluorimetric*
The secondary antibody and streptavidin conjugates of calf intestinal alkaline phosphatase are extensively used as secondary detection reagents in ELISA.
The SensoLyte® FDP Alkaline Phosphatase ELISA Assay Kit uses highly purified FDP to quantify alkaline phosphatase activity in ELISA. It has been used to detect as low as atto molar (10-15 M) concentration of test antibody in an ELISA format.
The kit contains: • FDP phosphatase substrate (Ex/Em=485±20/528±20 nm upon dephosphorylation) • Assay buffer • Stop solution • Wash buffer • Alkaline phosphatase-conjugated secondary antibody (goat anti-mouse IgG) • An optimized ELISA protocol